Antibodies, Assay Kits, Bap1 Antibody, Biology Cells, cDNA, Culture Cells, Devices, DNA Templates, DNA Testing, E coli, EIA, Eif2A Antibody, Elisa Kits, Enzymes, Exosomes, Fto Antibody, Hama Antibodies, Laminin Alpha 5, Muc2 Antibody, Nox1 Antibody, Panel, Particles, peroxidase, Pkr Antibody, plex, Positive, Premix, Preps, Primary Antibodies, primers, probe, Pure, Purification, purified, Rabbit, Reagents, Real-time, Recombinant Proteins, Rhesus, RNA, Test Kits, Zebrafish Antibodies

chromogen solution b

QuickDetect  Hydroxylysine (Human) ELISA Package
( Catalog # E4714-100 ; 96 assays ; Storage at 4ºC )
05/19
I. Introduction:
Hydroxylysine (Hyl) is an amino acid which arises from a post-translational hydroxy modification of lysine. It’s most generally referred to as a
part of collagen. QuickDetect™ Hydroxylysine (Human) ELISA Package makes use of a double-antibody sandwich enzyme-linked immunosorbent one-step course of assay to assay the extent of hydroxylysine in samples.
Commonplace, take a look at pattern and HRP-labeled hydroxylysine (antibodies had been added to enzyme wells that are Pre-coated with hydroxylysine antibody, then perform incubation and wash to take away the uncombined enzyme.

Upon including Chromogen Answer A and B, the colour of the liquid will develop into blue, and the response with the acid will trigger the colour to change into yellow. The depth of colour and the focus of the hydroxylysine pattern are positively correlated.

II. Functions:
• This ELISA package is used for in vitro quantitative dedication of Human Hydroxylysine
• Assay vary: 9.3ng/ml- 300ng/ml
• Accuracy: Commonplace linear regression correlation coefficient R with the anticipated worth of the focus, better than or equal to
0.9900.

III. Pattern Kind:
• Plasma
• Cell and tissue tradition supernatants
• Serum
• Different organic fluids
• Tissue and cell lysates

 

Summary

The alkylimidazolium tetrafluoroborate ionic liquids (ILs) ([Cnmim][BF4n = 2, 4, 6, 8, 10) and anionic surfactant sodium dodecyl sulfate (SDS) had been mixed collectively to provide efficient mediums for chromogenic catalysis of tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) within the presence of H2O2. The chromogenic efficiency, kinetic conduct, and the potential influencing mechanism for the chromogenic catalysis of HRP-H2O2-TMB had been mentioned intimately. Therein, the roles of ionic liquids (ILs) had been highlighted by the mixture of experiments and theoretical calculations. The SDS/[C4mim][BF4] mixture displayed superiority in chromogenic catalysis by bettering each the substrate solubility and product stability to the utmost extent potential. Moreover, SDS/[C4mim][BF4] mixture confirmed uniqueness for TMB in bettering the chromogenic efficiency in contrast with different chromogenic substrates of HRP. Impressed by the environment friendly chromogenic system, an enhanced enzyme-linked immunosorbent assay technique for the detection of human immunoglobulin G was established and the delicate colorimetric methods for the detection of H2O2 and glucose had been additional developed by using SDS/[C4mim][BF4] mixture because the medium of chromogenic catalysis of HRP-H2O2-TMB. This distinctive chromogenic system is endowed with multitude of potential purposes in organic methods.

chromogen solution b
chromogen resolution b

Introduction

Peroxidases are a big household of enzymes that usually catalyze the oxidation of their substrates with peroxide (H2O2 normally). (1) Horseradish peroxidase (HRP) is a very powerful heme-containing peroxidase discovered within the roots of horseradish. (2) HRP has been broadly utilized in bioanalytical and medical chemistry due to the comparatively steady properties, low-cost manufacturing, efficient catalytic exercise, in addition to wider number of substrates than different oxidoreductive enzymes. (3−6) It’s price mentioning that the HRP has potential purposes in enzyme-linked immunosorbent assay (ELISA), (3) electrochemical immunosensors, (7) in addition to different biosensors (8) for analysis and biochemical detection as a result of it may possibly catalyze numerous fragrant amine and phenolic compounds oxidation within the presence of H2O2 to provide a chromogenic response.
The earlier examine has proven that the chromogenic sensitivity varies with the chromogenic substrates used within the detection. It’s reported that tetramethylbenzidine (TMB) has the very best sensitivity to HRP in comparison with different chromogenic substrates reminiscent of o-phenylenediamine (OPD), 2,2-diazo-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 5-aminosalicylic acid (5-AS) and so forth. (1,9) Other than decrease toxicity and nonteratogenicity, (10) TMB shares some great benefits of splendid affinity with the enzyme and the chromogen with excessive absorption coefficient, and thus turns into an optimum substrate of HRP. (10,11) Nonetheless, some shortcomings of TMB affected by poor water solubility and quick colour growing time derived from the extremely unstable chromogens restrict the scope of its sensible software to a terrific diploma. (10,12,13) Due to this fact, growing a chromogenic catalysis system of HRP-H2O2-TMB with greater detection effectivity continues to be the objective pursued by researchers.

Beta III Tubulin Polyclonal Antibody

ABP50179-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide

Beta III Tubulin Polyclonal Antibody

ABP50179-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide

Beta III Tubulin Polyclonal Antibody

ABP50179-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide

Beta III tubulin Monoclonal Antibody

ABM40165-003ml 0.03ml
EUR 189.6
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

Beta III tubulin Monoclonal Antibody

ABM40165-01ml 0.1ml
EUR 346.8
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

Beta III tubulin Monoclonal Antibody

ABM40165-02ml 0.2ml
EUR 496.8
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

Beta III Tubulin

E8R1401-3 200ul
EUR 275
Description: Available in various conjugation types.

Tubulin beta-III Antibody

21617-100ul 100ul
EUR 302.4

Tubulin beta-III Antibody

21617-50ul 50ul
EUR 224.4

Tubulin beta-III Antibody

48701-100ul 100ul
EUR 399.6

Tubulin beta-III Antibody

48701-50ul 50ul
EUR 286.8

Tubulin Beta 3 Class III (TUBB3) Antibody

20-abx159746
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Tubulin Beta 3 Class III (TUBB3) Antibody

20-abx159747
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Tubulin Beta 3 Class III (TUBB3) Antibody

20-abx159748
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Tubulin Beta 3 Class III (TUBB3) Antibody

20-abx159749
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Tubulin Beta 3 Class III (TUBB3) Antibody

20-abx159750
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Tubulin Beta 3 Class III (TUBB3) Antibody

20-abx159751
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Tubulin Beta 3 Class III (TUBB3) Antibody

abx159752-100ul 100 ul
EUR 427.2

Anti-Beta III Tubulin Antibody

R1401-3 100ul
EUR 189
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. It has been reported that β3-tubulin is expressed in a wide variety of tumors. Overexpression of this isotype in clinical samples correlates with tumor aggressiveness, resistance to chemotherapeutic drugs, and poor patient survival. The β3 isotype increases tumor aggressiveness by two distinct mechanisms. Incorporation of this isotype makes microtubule networks hypostable, allowing them to resist the cytotoxic effects of microtubule stabilizing drugs like taxanes or epothilones. Mechanistically, it was found that overexpression of β3-tubulin increases the rate of microtubule detachment from microtubule organizing centers, an activity that is suppressed by drugs such as paclitaxel.

Anti-Beta III Tubulin Antibody

0805-3 100ul
EUR 189
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.

Anti-Beta III Tubulin Antibody

0807-2 100ul
EUR 189
Description: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.

Anti-Beta III Tubulin antibody

STJ16100477 100 µg
EUR 487.2

Anti-beta III tubulin antibody

STJ97067 200 µl
EUR 236.4
Description: Mouse monoclonal to beta III tubulin.

Anti-beta III Tubulin antibody

STJ97123 200 µl
EUR 236.4
Description: Beta III tubulin is a protein encoded by the tubb3 gene which is approximately 50,4 kDa. Beta III tubulin is localised to the cytoplasm and cytoskeleton. It is involved in signalling within gap junctions, development slit-robo signalling, sertoli-sertoli cell junction dynamics, chaperonin-mediated protein folding and tubulin folding. This protein falls under the one of two protein families, namely beta and alpha tubulin and is one of six beta tubulin isoforms. Beta III tubulin is expressed in the neurons of the peripheral and central nervous system and plays an important role in guidance and maintenance and axons. Mutations in the tubb3 gene result in congenital fibrosis of extraocular muscles type. STJ97123 was developed from clone Mix. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta III tubulin protein.

Tubulin beta-III Conjugated Antibody

C48701 100ul
EUR 476.4

Tubulin beta-III Conjugated Antibody

C21617 100ul
EUR 476.4

beta III Tubulin Rabbit pAb

E2380630 100ul
EUR 225
Description: Available in various conjugation types.

beta III Tubulin Rabbit pAb

E2380631 100ul
EUR 225
Description: Available in various conjugation types.

beta III Tubulin Rabbit pAb

E2380632 100ul
EUR 225
Description: Available in various conjugation types.

beta III Tubulin Rabbit mAb

E2R23618 100ul
EUR 255
Description: Available in various conjugation types.

beta III Tubulin Rabbit mAb

E2R23620 100ul
EUR 255
Description: Available in various conjugation types.

Tubulin beta-III

E8ET1604-17 100ul
EUR 275
Description: Available in various conjugation types.

anti-Neuron specific beta III Tubulin

YF-PA16915 100 ug
EUR 483.6
Description: Rabbit polyclonal to Neuron specific beta III Tubulin

Anti-Beta III Tubulin Antibody [A8-D10]

M0805-8 100ul
EUR 210
Description: Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.

Anti-Beta III Tubulin Antibody [SP06-00]

ET1604-17 100ul
EUR 231
Description: Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

beta Tubulin III Rabbit mAb

A17913 20 μL
EUR 18

beta Tubulin III Rabbit mAb

A17913-100ul 100 ul
EUR 492

beta Tubulin III Rabbit mAb

A17913-200ul 200 ul
EUR 685.2