chromogen solution b
QuickDetect Hydroxylysine (Human) ELISA Package
( Catalog # E4714-100 ; 96 assays ; Storage at 4ºC )
05/19
I. Introduction:
Hydroxylysine (Hyl) is an amino acid which arises from a post-translational hydroxy modification of lysine. It’s most generally referred to as a
part of collagen. QuickDetect™ Hydroxylysine (Human) ELISA Package makes use of a double-antibody sandwich enzyme-linked immunosorbent one-step course of assay to assay the extent of hydroxylysine in samples.
Commonplace, take a look at pattern and HRP-labeled hydroxylysine (antibodies had been added to enzyme wells that are Pre-coated with hydroxylysine antibody, then perform incubation and wash to take away the uncombined enzyme.
Upon including Chromogen Answer A and B, the colour of the liquid will develop into blue, and the response with the acid will trigger the colour to change into yellow. The depth of colour and the focus of the hydroxylysine pattern are positively correlated.
II. Functions:
• This ELISA package is used for in vitro quantitative dedication of Human Hydroxylysine
• Assay vary: 9.3ng/ml- 300ng/ml
• Accuracy: Commonplace linear regression correlation coefficient R with the anticipated worth of the focus, better than or equal to
0.9900.
III. Pattern Kind:
• Plasma
• Cell and tissue tradition supernatants
• Serum
• Different organic fluids
• Tissue and cell lysates
Summary
The alkylimidazolium tetrafluoroborate ionic liquids (ILs) ([Cnmim][BF4] n = 2, 4, 6, 8, 10) and anionic surfactant sodium dodecyl sulfate (SDS) had been mixed collectively to provide efficient mediums for chromogenic catalysis of tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) within the presence of H2O2. The chromogenic efficiency, kinetic conduct, and the potential influencing mechanism for the chromogenic catalysis of HRP-H2O2-TMB had been mentioned intimately. Therein, the roles of ionic liquids (ILs) had been highlighted by the mixture of experiments and theoretical calculations. The SDS/[C4mim][BF4] mixture displayed superiority in chromogenic catalysis by bettering each the substrate solubility and product stability to the utmost extent potential. Moreover, SDS/[C4mim][BF4] mixture confirmed uniqueness for TMB in bettering the chromogenic efficiency in contrast with different chromogenic substrates of HRP. Impressed by the environment friendly chromogenic system, an enhanced enzyme-linked immunosorbent assay technique for the detection of human immunoglobulin G was established and the delicate colorimetric methods for the detection of H2O2 and glucose had been additional developed by using SDS/[C4mim][BF4] mixture because the medium of chromogenic catalysis of HRP-H2O2-TMB. This distinctive chromogenic system is endowed with multitude of potential purposes in organic methods.
Introduction
Beta III Tubulin Antibody (TUBB3) |
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F49314-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. |
beta III Tubulin Conjugated Antibody |
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C48012 | SAB | 100ul | EUR 476.4 |
Beta III Tubulin Polyclonal Antibody |
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ABP50179-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide |
Beta III Tubulin Polyclonal Antibody |
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ABP50179-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide |
Beta III Tubulin Polyclonal Antibody |
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ABP50179-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of Beta III Tubulin from Human, Mouse, Rat. This Beta III Tubulin antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide |
Beta III tubulin Monoclonal Antibody |
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ABM40165-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide |
Beta III tubulin Monoclonal Antibody |
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ABM40165-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide |
Beta III tubulin Monoclonal Antibody |
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ABM40165-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A monoclonal antibody for detection of Beta III tubulin from Human, Mouse, Rat. This Beta III tubulin antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide |
OASL01038-100UL - Beta III Tubulin Antibody |
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OASL01038-100UL | Aviva Systems Biology | 100ul | EUR 269 |
Tubulin beta-III Antibody |
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21617-100ul | SAB | 100ul | EUR 302.4 |
Tubulin beta-III Antibody |
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21617-50ul | SAB | 50ul | EUR 224.4 |
Tubulin beta-III Antibody |
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48701-100ul | SAB | 100ul | EUR 399.6 |
Tubulin beta-III Antibody |
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48701-50ul | SAB | 50ul | EUR 286.8 |
Tubulin beta-III Antibody |
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48701 | SAB | 100ul | EUR 499 |
Tubulin beta-III Antibody |
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21617 | SAB | 100ul | EUR 319 |
Beta III Tubulin |
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E8R1401-3 | EnoGene | 200ul | EUR 275 |
Description: Available in various conjugation types. |
Tubulin Beta 3 Class III (TUBB3) Antibody |
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20-abx159746 | Abbexa |
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Tubulin Beta 3 Class III (TUBB3) Antibody |
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20-abx159747 | Abbexa |
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Tubulin Beta 3 Class III (TUBB3) Antibody |
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20-abx159748 | Abbexa |
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Tubulin Beta 3 Class III (TUBB3) Antibody |
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20-abx159749 | Abbexa |
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Tubulin Beta 3 Class III (TUBB3) Antibody |
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20-abx159750 | Abbexa |
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Tubulin Beta 3 Class III (TUBB3) Antibody |
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20-abx159751 | Abbexa |
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Tubulin Beta 3 Class III (TUBB3) Antibody |
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abx159752-100ul | Abbexa | 100 ul | EUR 427.2 |
beta III Tubulin (TUBB3) chicken polyclonal antibody |
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CH23005-100 | Origene Technologies GmbH | 100 µl | Ask for price |
Anti-Beta III Tubulin antibody |
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STJ16100477 | St John's Laboratory | 100 µg | EUR 487.2 |
Anti-beta III tubulin antibody |
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STJ97067 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to beta III tubulin. |
Anti-beta III Tubulin antibody |
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STJ97123 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Beta III tubulin is a protein encoded by the tubb3 gene which is approximately 50,4 kDa. Beta III tubulin is localised to the cytoplasm and cytoskeleton. It is involved in signalling within gap junctions, development slit-robo signalling, sertoli-sertoli cell junction dynamics, chaperonin-mediated protein folding and tubulin folding. This protein falls under the one of two protein families, namely beta and alpha tubulin and is one of six beta tubulin isoforms. Beta III tubulin is expressed in the neurons of the peripheral and central nervous system and plays an important role in guidance and maintenance and axons. Mutations in the tubb3 gene result in congenital fibrosis of extraocular muscles type. STJ97123 was developed from clone Mix. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta III tubulin protein. |
Anti-Beta III Tubulin Antibody |
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R1401-3 | HUABIO | 100ul | EUR 189 |
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. It has been reported that β3-tubulin is expressed in a wide variety of tumors. Overexpression of this isotype in clinical samples correlates with tumor aggressiveness, resistance to chemotherapeutic drugs, and poor patient survival. The β3 isotype increases tumor aggressiveness by two distinct mechanisms. Incorporation of this isotype makes microtubule networks hypostable, allowing them to resist the cytotoxic effects of microtubule stabilizing drugs like taxanes or epothilones. Mechanistically, it was found that overexpression of β3-tubulin increases the rate of microtubule detachment from microtubule organizing centers, an activity that is suppressed by drugs such as paclitaxel. |
Anti-Beta III Tubulin Antibody |
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0805-3 | HUABIO | 100ul | EUR 189 |
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
Anti-Beta III Tubulin Antibody |
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0807-2 | HUABIO | 100ul | EUR 189 |
Description: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP. |
beta III Tubulin (TUBB3) (tubulin beta-3 chain) mouse monoclonal antibody, clone 3D9, Purified |
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AM10229PU-N | Origene Technologies GmbH | 100 µg | Ask for price |
Tubulin beta-III Conjugated Antibody |
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C48701 | SAB | 100ul | EUR 476.4 |
beta III Tubulin Rabbit pAb |
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E2380630 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
beta III Tubulin Rabbit pAb |
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E2380631 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
beta III Tubulin Rabbit pAb |
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E2380632 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
beta III Tubulin Rabbit mAb |
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E2R23618 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
beta III Tubulin Rabbit mAb |
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E2R23620 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
beta III Tubulin Rabbit mAb |
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52177 | SAB | 100ul | EUR 499 |