Recombinant Proteinase K, Molecular Biology Grade

Proteinase Okay – one among many usually used enzymes in molecular biology.

Wonderful completely different for protein digestion in assorted options.

  • BLIRT – expert in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing all through the best top of the range product with a really low batch-to-batch variability.
  • Enormous components, the possibility of white labeling, and portioning service – liquid selection all through the quantity on the shopper’s request (as lots as quite a lot of million samples month-to-month).

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase Okay (Lyophilized)

For Basic Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Simple to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as lots as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Bear in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in path of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Secure over a giant pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the simplest top of the range product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by way of nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Proteinase K, recombinant
Proteinase K, recombinant

Selections

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as lots as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
  • Secure over a giant pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from fully completely completely different beginning offers.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by way of nucleic acids isolation.

 

Proteinase K
BIO-37037 100mg Ask for price

Proteinase K
BIO-37039 1000mg Ask for price

Proteinase K
Z5050002 100 mg
EUR 172

Proteinase K
MB086-100MG 1 unit
EUR 71.16
Description: Proteinase K

Proteinase K
MB086-10MG 1 unit
EUR 7.75
Description: Proteinase K

Proteinase K
MB086-1G 1 unit
EUR 554.5
Description: Proteinase K

Proteinase K
RM2957-100MG 1 unit
EUR 93.9
Description: Proteinase K

Proteinase K
RM2957-10MG 1 unit
EUR 10.43
Description: Proteinase K

Proteinase K
RM2957-1G 1 unit
EUR 844.84
Description: Proteinase K

Proteinase K
EQ023-1mL 1mL
EUR 16

Proteinase K
E4350-01 1ml
EUR 23.98
Description: Solution 20 mg/ml

Proteinase K
E4350-02 5x1ml
EUR 107.91
Description: Solution 20 mg/ml

Proteinase K
GE0990 25mg
EUR 347.58

Proteinase K
G029 1.0 ml
EUR 85
Description: Proteinase K is a serine protease often used to digest protein and to remove contamination in nucleic acid preparations. It does so by inactivating nucleases which could otherwise cause the break down of DNA and/or RNA.

Proteinase K
EQ023 1mL
EUR 16

PROTEINASE K
IB05402 200MG
EUR 158.37
Description: (FR)

Proteinase K
GE0990-1 1
EUR 379.6

Proteinase K
GE0990-100 100
EUR 63.3

Proteinase K
GE0990-100MG 100 mg
EUR 112.8