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Protein A, HRP conjugate

PROPERTIES

producer/tradename

Upstate®

software(s)

ELISA: appropriate
dot blot: appropriate
immunohistochemistry: appropriate
western blot: appropriate

shipped in

moist ice

DESCRIPTION

Software

Appropriate for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.

Bodily kind

chromatographically pure Protein A conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10 mg/ml BSA- Immunoglobulin and Protease free

Storage and Stability

Lyophilized: Secure for two years at -20°C from date of cargo. Rehydrated: Secure for six months at -20°C.

Authorized Data

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Until in any other case acknowledged in our catalog or different firm documentation accompanying the product(s), our merchandise are meant for analysis use solely and will not be for use for another function, which incorporates however shouldn’t be restricted to, unauthorized industrial makes use of, in vitro diagnostic makes use of, ex vivo or in vivo therapeutic makes use of or any sort of consumption or software to people or animals.

Suggestions for Use of ab171537 HRP Stability Conjugate Reagent

  • For optimum stability and efficiency, use ab171537 at 100% focus
  • Dilute your HRP conjugate to optimum working focus within the HRP Stability Conjugate Reagent resolution
  • Use diluted conjugate working resolution in your assay as regular
  • Or, ideally, retailer away from direct mild at room temperature and use as a inventory resolution for optimum assay-to-assay consistency

NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent resolution for an prolonged time period: If a phosphate buffer is used for dilution we suggest a remaining focus of not more than 5 mM buffer to keep away from the potential of initiating precipitation.

HRP Conjugate Reagent
HRP Conjugate Reagent

Instantly label your major antibody with HRP

​​​Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which could be conjugated to antibodies and proteins to be used in a wide range of purposes.

The enzyme label could be visualized via chromogenic reactions. For instance, diaminobenzidine (DAB) within the presence of hydrogen peroxide (H202) is transformed right into a water-insoluble brown pigment. Different substrates that can be utilized to measure horseradish peroxidase exercise embrace ABTS, TMB, and TMBUS.

HRP is a well-liked detection label utilized in analysis. Antibody-HRP conjugates are generally utilized in ELISA, IHC, and western blotting. HRP could be conjugated to the first antibody for direct detection or secondary antibody for oblique detection.

Direct detection is usually a most well-liked methodology inside these purposes to keep away from cross-species reactivity and to eradicate extra laborious wash and separation steps, particularly in time-consuming protocols. Nonetheless, instantly conjugating HRP to the antibody or protein of selection could be tough and labor-intensive if utilizing conventional methodology.

Our Lightning-Hyperlink HRP antibody labeling equipment permits the direct conjugation of your antibody, protein, or peptide to HRP to be used in any software. The equipment solely requires 30 seconds of hands-on time, and conjugates are prepared to make use of after solely three hours. Moreover, there isn’t a want for additional wash or separation steps submit conjugation.

 

Human GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit

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Human GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit

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Human GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit

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Human GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit

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  • 100ug
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  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
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Description: A polyclonal antibody against GLI1. Recognizes GLI1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

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Description: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

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Description: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.