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Plant cell culture technologies

by Frank Rivera
Abstract

The industrial exploitation of plant cell, tissue and organ cultures is now a actuality and the brand new applied sciences are already in place and creating quickly. Their emergence has supplied new views and sharpened the main focus of the methods through which plant cell and tissue tradition can assist man. Along with the thrilling new developments in plant molecular biology, these in vitro procedures ought to enable plant bio technologists to ‘design’ crops and plant merchandise and exploit the total industrial potential of plant cell cultures.

PROPERTIES

High quality Stage

200

organic supply

algae (Rhodophyceae)

type

powder

utility(s)

cell tradition | mammalian: appropriate
cell tradition | plant: appropriate
microbiology: appropriate

transition temp

congealing temperature <38 °C (1.5% in H2O)

suitability

microbiology examined

Featured Business

Agriculture

storage temp.

room temp

DESCRIPTION

Common description

Agar is a hydrophilic colloid obtained from marine algae (Rhodophyceae). It’s soluble in boiling water and is of significance to the microbiological space.
Agar chemically consists of two fractions: agarose, a impartial polymer of excessive gel power and agaropectin, a non-gelling fraction with sulfated β-1,3-glycosidically linked D-galactose items.

Utility

Agar has been used:

  • as a reference commonplace industrial agar to match the physio-chemical, gelling properties of alkali-treated agar from Gracilaria tenuistipitata
  • as a part within the seed germination medium and rooting medium for commercially obtainable soybean (Glycine max (L.) Merr) seeds
  • as a part of development media for B. subtilis mutant pressure MTC871 based mostly biofilm colonies
  • as a bacteriological agar part to arrange half power Murashige-Skoog (MS 50%) medium for Echinocactus platycanthus seeds tradition
PPM
PPM
Agar has been used within the plant development media.
Typical working focus: 6-12g/L.

Packaging

5, 10, 25 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Biochem/physiol Actions

Agar is gel forming polysaccharide used as a gelling and stabilizing agent. Its serves as a cryoprotectant in processed meals, prescription drugs and cosmetics merchandise.

Different Notes

Common function agar.

Bacterial Ailments

Some micro organism inflicting infecting Hashish crops embrace Pseudomonas syringae and Xanthomonas campestris pv. Hashish. The signs of Pseudomonas syringae are small water-soaked leaf spots which will enlarge alongside the veins, turning brown. The Xanthomonas campestris causes leaf spots and wilting in crops.

Fungal Ailments

A few of the main hashish infecting fungus and ailments attributable to them are:

  • Fusarium oxysporum f.sp. Hashish causes wilt whose signs embrace yellowing of leaves, poor development, and wilt.
  • Pythium illness causes root rot and damping-off illness whose signs fluctuate from small roots lesions, extreme root injury, stunted development, to yellowing of leaves. Additional, damping-off impacts younger seedlings.
  • Sclerotinia sclerotiorum causes hemp canker whose preliminary signs embrace watersoaked lesions on stalks and branches that may later trigger cankers. Generally cottony white mycelium and black sclerotia may seem.
  • Sphaeorotheca macularis or Leveillula taurica causes powdery mildew. It’s one of the crucial widespread foliar ailments of hashish. Its signs embrace powdery development on the floor of leaves that later turns brown.
  • The hashish plant can be affected by Alternaria species, which causes leaf spot and brown blight ailments.

Viral and Viroid Ailments

Viruses affecting hashish embrace Hop mosaic virus (HpMV), Apple mosaic virus (ApMV), Hop stunt viroid (HSVd), Hop latent viroid, Arabis mosaic virus (ArMV), Alfalfa mosaic virus (AMV), Tobacco mosaic virus (TMC), Cucumber mosaic virus (CMV), and phytoplasmas.

The viruses may cause extreme crop losses, cut back development, or have an effect on the yield and high quality of crops. The widespread signs of viruses embrace yellow and inexperienced mosaic patterns on the leaves of Hashish and curling, distortion, and narrowing of younger leaves. Phytoplasmas primarily trigger extreme shoot proliferation and stunted development.

 

CD273 Recombinant Protein
RF16020-01 0.1 mg
EUR 619.8
Description: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Mouse Anti-Human CD273 (PD-L2) mAbConjugated Antibody
CCM031 100ul
EUR 476.4

Anti-Hu CD273 PE
1P-178-T025 25 tests
EUR 168

Anti-Hu CD273 PE
1P-178-T100 100 tests
EUR 288

Anti-Hu CD273 APC
1A-178-T100 100 tests
EUR 288

Anti-Hu CD273 Purified
11-178-C025 0.025 mg
EUR 118.8

Anti-Hu CD273 Purified
11-178-C100 0.1 mg
EUR 189.6

Mouse Anti-Human CD273 (PD-L2) mAb
CM031-100ug 100ug
EUR 250.8

Mouse Anti-Human CD273 (PD-L2) mAb
CM031-25ug 25ug
EUR 154.8

Anti-Hu CD273 Alexa Fluor647
A6-178-T100 100 tests
EUR 322.8

CD273 [PD-L2] Recombinant Protein
90-425 100 ug
EUR 651.3
Description: T cells require a signal induced by the engagement of the T cell receptor and a costimulatory signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. CD273 (PD-L2) is one of two ligands for programmed death-1 (PD-1; CD279), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. CD273 is broadly expressed and also up regulated in a variety of tumor cell lines. On previously activated T cells, CD273 interaction with PD-1 inhibits TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. CD273 has a costimulatory function on resting T cells activated with suboptimal TCR signals.

CD273 [PD-L2] Recombinant Protein
90-427 100 ug
EUR 651.3
Description: T cells require a signal induced by the engagement of the T cell receptor and a costimulatory signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. CD273 (PD-L2) is one of two ligands for programmed death-1 (PD-1; CD279), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. CD273 is broadly expressed and also up regulated in a variety of tumor cell lines. On previously activated T cells, CD273 interaction with PD-1 inhibits TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. CD273 has a costimulatory function on resting T cells activated with suboptimal TCR signals.

CD273 [PD-L2] Recombinant Protein
90-428 100 ug
EUR 651.3
Description: T cells require a signal induced by the engagement of the T cell receptor and a costimulatory signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. CD273 (PD-L2) is one of two ligands for programmed death-1 (PD-1; CD279), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. CD273 is broadly expressed and also up regulated in a variety of tumor cell lines. On previously activated T cells, CD273 interaction with PD-1 inhibits TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. CD273 has a costimulatory function on resting T cells activated with suboptimal TCR signals.

Anti-Hu CD273 PE-Cy7
T7-178-T100 100 tests
EUR 321.6

Recombinant Mouse PD-L2/B7-DC/CD273 Protein
RP00660 10 μg
EUR 174

PD-L2 (CD273), Fc fusion (Human)
71107 100 µg
EUR 330
Description: Human secreted Programmed Death Ligand 2 (PD-L2)-Fc fusion protein, also known as CD273, PDCDL1G2, and B7-DC, GenBank Accession No. NM_025239, a.a. 20-219, fused at the C-terminus to the Fc portion of human IgG1 expressed in a HEK293 cell expression system. MW = 49 kDa.

Programmed Cell Death 1 Ligand 2 (CD273) Antibody
abx140405-01mg 0.1 mg
EUR 427.2

Programmed Cell Death 1 Ligand 2 (CD273) Antibody
abx414420-02mg 0.2 mg
EUR 678

Programmed Cell Death 1 Ligand 2 (CD273) Antibody
abx414421-01mg 0.1 mg
EUR 526.8

Programmed Cell Death 1 Ligand 2 (CD273) Antibody
abx412031-01mg 0.1 mg
EUR 610.8

Programmed Cell Death 1 Ligand 2 (CD273) Antibody
abx414235-025mg 0.25 mg
EUR 678

Anti-CD273/ PD-L2 Antibody [TY25], Unconjugated-100ug
QAB101-100ug 100ug
EUR 270

PD-L2 Antibody / Programmed death ligand 2 / CD273
R32447 100 ug
EUR 419

PD-L2 Antibody / Programmed death ligand 2 / CD273
V8176-100UG 100 ug
EUR 499
Description: Recognizes a protein of about 31kDa, which is identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.

PD-L2 Antibody / Programmed death ligand 2 / CD273
V8176-20UG 20 ug
EUR 219
Description: Recognizes a protein of about 31kDa, which is identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.

PD-L2 Antibody / Programmed death ligand 2 / CD273
V8176SAF-100UG 100 ug
EUR 499
Description: Recognizes a protein of about 31kDa, which is identified as PD-L2 (same as PDCD1LG2). Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. PD-L2 does not bind CD28, cytotoxic T lymphocyte A4 or ICOS (inducible co-stimulator). The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.

PD-L2 Antibody / Programmed death ligand 2 / CD273
V7546-100UG 100 ug
EUR 499
Description: PD-L2/PDCD1LG2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt]

PD-L2 Antibody / Programmed death ligand 2 / CD273
V7546-20UG 20 ug
EUR 219
Description: PD-L2/PDCD1LG2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt]