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Retro-orbital injection of FITC-dextran combined with isolectin B4 in assessing the retinal neovascularization defect

by Frank Rivera
Background: A dependable and efficient methodology is required to ship agent that may support the in vivo imaging of retinal vessels. The intention of the current examine was to guage retro-orbital (RO) injection of fluorescein-labeled isothiocyanate dextran (FITC-dextran) as a technique of demonstrating retinal neovascularization (NV) and avascular areas in oxygen-induced retinopathy (OIR) mice.
Strategies: Totally different concentrations of FITC-dextran have been used to match the efficacy of this agent in perfusing the retinal vessels. Hematoxylin-eosin (HE) staining was used to guage the protection of RO injection. The vitreous blood vessels and extent of NV have been assessed in P17 OIR mice utilizing FITC-dextran and in contrast with the corresponding measurements obtained following isolectin B4 staining or the mix of each strategies.
Outcomes: The fluorescence of small vessels and neovascular tufts may very well be noticed clearly following RO injection of 0.05 ml of 25 mg/ml or 50 mg/ml FITC-dextran. No seen harm to tissues adjoining to the injection web site was found. Vitreous blood stream was steadily decreased from P0 to P5 and finally disappeared in P17 OIR mice, as demonstrated by FITC-dextran perfusion.
The retinal NV areas assessed by isolectin B4 have been bigger than these assessed by FITC-dextran, however the retinal avascular areas have been smaller. The mixture of each strategies might conduce to differentiate non-functional blood vessels.
Conclusions: RO injection of FITC-dextran mixed with isolectin B4 is an efficient, optimum methodology for assessing the NV space and avascular space.

Analysis Notice: Modified serum fluorescein isothiocyanate dextran (FITC-d) assay process to find out intestinal permeability in poultry fed diets excessive in man-made or natural pigments

Oral administration of fluorescein isothiocyanate dextran (FITC-d) has been used as an indicator for intestinal permeability in poultry analysis for a number of years. Below wholesome situations, tight junctions within the intestinal wall won’t permit the 4-6kDa FITC-d to enter the bloodstream. Detection of FITC-d in serum (1-hour post-oral administration of FITC-d) has confirmed to be a dependable indicator of leaky intestine syndrome (elevated intestinal irritation and disruption of tight junctions). Administration of supplementary phytobiotics in feed, notably merchandise with excessive beta-carotene ranges or different pigments, has resulted in robust serum background fluorescence, which might render this assay unreliable.
To account for this enhance in background autofluorescence, the FITC-d assay process has been modified to accommodate these specific serum samples by together with pre-administration serum assortment from every therapy group to take away background fluorescence. The modified FITC-d process detailed will permit for evaluation of intestinal permeability in pigmented serum.

 

Chemical conjugation of FITC to trace silica nanoparticles in vivo and in vitro: An rising methodology to evaluate the reproductive toxicity of business nanomaterials

 

Earlier research have demonstrated that silica nanoparticle (SiNP) publicity induces pulmonary and cardiovascular illnesses, but their transportation and degradation in vivo haven’t been absolutely elucidated. From the attitude of replica, this examine was carried out to look at the uterine accumulation of SiNP and discover its reproductive toxicity and pathogenic mechanisms.
  • First, we coupled FITC onto SiNPs and intratracheally instilled them into pregnant mice on the fifth gestational day, and the poisonous impact of SiNP was evaluated in vitro and in vivo. It was discovered that SiNP penetrated the trophoblast membrane, resulting in apoptosis and suppression of cell proliferation, tube formation, and invasion in a dose-dependent method.
  • Mechanistically, SiNP dysregulated the expression of Scd1, Slc27a1, and Cpt1a, and induced over synthesis and efflux obstruction of fatty acid by the PPARγ signaling pathway. The downregulation of Caspase-Three triggered apoptosis of trophoblast, which was causally related to intracellular fatty acid accumulation as revealed by the correlation evaluation.
  • In addition to, SiNP induced uterine irritation in vivo, which aggravated with the remark prolongation inside 24 h. General, SiNPs have been visualized by coupling with FITC, and the uterine accumulation of SiNP induced fatty acid metabolic dysfunction, organic dysfunction, and trophoblast apoptosis, which have been mediated partially by the PPARγ signaling pathway. These findings would contribute to understanding the environmental impacts of SiNP higher, in addition to the event of management measures for environmental air pollution.

An Annexin V-FITC-Propidium Iodide-Primarily based Technique for Detecting Apoptosis in a Non-Small Cell Lung Most cancers Cell Line

Annexin V and propidium iodide staining is broadly used for figuring out the mobile demise by apoptosis. Within the presence of Ca2+ ions, annexin V has a powerful binding affinity for phosphatidylserine, a membrane phospholipid that in apoptosis is translocated from the inside facet of the cell membrane to its outer facet. Then again, propidium iodide has means for DNA binding and it might probably solely enter into necrotic or late apoptotic cells.
This chapter describes a generally used methodology for detection of apoptosis in a non-small cell lung most cancers cell line utilizing annexin V and propidium iodide dye. We describe the detection of various phases of apoptosis within the A549 lung most cancers cell line handled with dihydroartemisinin (DHA). This apoptosis detection methodology can be utilized to find out the efficacy of various varieties of medication on cultured most cancers cell strains.
teitell-lab
teitell-lab

FITC-Dextran Launch from Cell-Embedded Fibrin Hydrogels

 

Fibrin hydrogel is a central organic materials in tissue engineering and drug supply purposes. As such, fibrin is often mixed with cells and biomolecules focused to the regenerated tissue. Earlier research have analyzed the discharge of various molecules from fibrin hydrogels; nevertheless, the impact of embedded cells on the discharge profile has but to be quantitatively explored.
This examine targeted on the discharge of Fluorescein isothiocyanate (FITC)-dextran (FD) 250 kDa from fibrin hydrogels, populated with completely different concentrations of fibroblast or endothelial cells, throughout a 48-h remark interval. The addition of cells to fibrin gels decreased the general launch by a small share (by 7-15% for fibroblasts and 6-8% for endothelial cells) relative to acellular gels.
The discharge profile was proven to be modulated by numerous mobile actions, together with gel degradation and bodily obstruction to diffusion. Cell-generated forces and matrix deformation (i.e., densification and fiber alignment) weren’t discovered to considerably affect the discharge profiles. This data is anticipated to enhance fibrin integration in tissue engineering and drug supply purposes by enabling predictions and methods to modulate the discharge profiles of varied biomolecules.

Uterine metabolic dysfunction induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC

 

Intensive software of nanomaterials has dramatically elevated the danger of silica nanoparticle (SiNP, SiO2) publicity, but their organic impact on replica has not been absolutely elucidated. By monitoring the uterine biodistribution of SiNP in pregnant mice, this examine was performed to guage the organic impact of SiNP on replica.
First, SiNP was conjugated with FITC, after which the FITC-SiNP was administrated to trophoblast (100 µg/mL, 24 h) in vitro and pregnant mice (0.25 mg/mouse, 2-24 h) in vivo. It was discovered that the FITC-SiNP was internalized by trophoblast and deposited within the uterus. The internalization of SiNP precipitated trophoblast dysfunction and apoptosis, whereas SiNP accumulation within the uterus induced diffuse inflammatory infiltration.
The genome-wide alteration of gene expression was studied by excessive throughput sequencing evaluation, the place 75 genes have been discovered to be dysregulated after SiNP publicity, amongst which ACOT2, SCD1, and CPT1A have been demonstrated to manage the biosynthesis of unsaturated fatty acids. Furthermore, the suppression of unsaturated fatty acids precipitated mitochondrial overload of long-chain fatty acyl-CoA (LACoA), which additional induced each trophoblast apoptosis and endometrial irritation.
In conclusion, the profitable conjugation of FITC onto SiNP facilitated the monitoring of SiNP in vitro and in vivo, whereas publicity to FITC-SiNP induced uterine metabolic dysfunction, which was regulated by the ACOT/CPT1A/SCD1 axis by the biosynthesis of unsaturated fatty acids signaling pathway.

Tie-2 Antibody
45046-50ul 50ul
EUR 224.4

Rabbit Anti-human TIE-1 antiserum #2
TIE12-S 100 ul
EUR 548.4

Tie-2/ Rat Tie- 2 ELISA Kit
ELA-E0126r 96 Tests
EUR 1063.2

Tie- 2 Antibody
ABD7500 100 ug
EUR 525.6

Rabbit Anti-human TIE-2 IgG #2, aff pure
TIE22-A 100 ug
EUR 578.4

TEK/Tie-2 ELISA KIT|Human
EF007833 96 Tests
EUR 826.8

Human TIE-2 Control/blocking peptide #2
TIE22-P 100 ug
EUR 196.8

Recombinant Human TIE-2 Fc Chimera
7-04189 1µg Ask for price

Recombinant Human TIE-2 Fc Chimera
7-04190 3µg Ask for price

Recombinant Human TIE-2 Fc Chimera
7-04191 100µg Ask for price

Tie-2 Conjugated Antibody
C45046 100ul
EUR 476.4

Tie-2 Polyclonal Antibody
ABP56367-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Tie-2 from Human, Mouse. This Tie-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Tie-2 around the non-phosphorylation site of Y1102

Tie-2 Polyclonal Antibody
ABP56367-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Tie-2 from Human, Mouse. This Tie-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Tie-2 around the non-phosphorylation site of Y1102

Tie-2 Polyclonal Antibody
ABP56367-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Tie-2 from Human, Mouse. This Tie-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Tie-2 around the non-phosphorylation site of Y1102

Tie-2 Polyclonal Antibody
ABP56368-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Tie-2 from Human, Mouse. This Tie-2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Tie-2 around the non-phosphorylation site of Y992

Tie-2 Polyclonal Antibody
ABP56368-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Tie-2 from Human, Mouse. This Tie-2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Tie-2 around the non-phosphorylation site of Y992

Tie-2 Polyclonal Antibody
ABP56368-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Tie-2 from Human, Mouse. This Tie-2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Tie-2 around the non-phosphorylation site of Y992

Tie-2 Polyclonal Antibody
ES7366-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Tie-2 from Human/Mouse. This antibody is tested and validated for IHC, WB, ELISA, WB, ELISA

Tie-2 Polyclonal Antibody
ES7366-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Tie-2 from Human/Mouse. This antibody is tested and validated for IHC, WB, ELISA, WB, ELISA

Tie-2 Polyclonal Antibody
ES7367-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Tie-2 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Tie-2 Polyclonal Antibody
ES7367-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Tie-2 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Nori® Human Tie-2 ELISA Kit
GR106458 96-well
EUR 461

Rabbit Anti-human TIE-1 IgG #2, aff pure
TIE12-A 100 ug
EUR 578.4

Rabbit Anti-human TIE-2 IgG #1 aff pure
TIE21-A 100 ug
EUR 578.4

Recombinant (Sf9) Human TIE-2 Fc Chimera
RP-705 10 ug
EUR 343.2

Monoclonal Anti-human TIE-2 IgG # 3, aff pure
TIE23-M 100 ug
EUR 578.4

Human TIE-1 Control/blocking peptide #2
TIE12-P 100 ug
EUR 196.8

Human TIE-2 Control/blocking peptide #1
TIE21-P 100 ug
EUR 196.8