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FITC characterization of a cathepsin B-responsive nanoprobe for report of differentiation of HL60 cells into macrophages

A cathepsin B (Cat B)-responsive optical nanoprobe is designed and ready for report of HL60 differentiation into macrophage. A peptide sequence FRFK is linked to fluorescein (FITC) through the distant amino group of its lysine and N-terminated with acrylic acid (AA) to yield a molecular fluorescent probe AA-FRFK (FITC). The molecular probe is additional embedded in poly(lactic-co-glycolic acid) (PLGA) to kind a fluorescent nanoprobe AA-FRFK (FITC)@PLGA.
The resultant optical nanoprobe is degradable by lysosomal Cat B, which is expressed in macrophages with a degree of 5-10 instances of that in HL60 cells. Consequently, a big lower in fluorescence depth is related to the differentiation means of HL60 to macrophage and can be utilized as a sign of the differentiation course of.
The findings could pave a approach towards the event of a common in vitro labeling technique of exogenous stem cells for report of in vivo cell differentiation by a dual-mode imaging modality involving optical imaging and magnetic resonance imaging.

An inhibitory immunoreceptor, Allergin-1, suppresses FITC-induced sort 2 contact hypersensitivity

Though allergic contact dermatitis (ACD) is the most typical T cell-mediated inflammatory responses towards an allergen within the pores and skin, the pathogenesis of ACD stays incompletely understood. Within the sensitization section in ACD, hapten-bearing dermal dendritic cells (DCs) play a pivotal function within the transport of an antigen to the lymph nodes (LNs), the place they current the antigen to naïve T cells. Right here we report that Allergin-1, an inhibitory immunoreceptor containing immunoreceptor tyrosine-based inhibitory motif (ITIM) within the cytoplasmic area, is extremely expressed on dermal DCs.
Mice poor in Allergin-1 exhibited exacerbated fluorescein isothiocyanate (FITC)-induced sort 2 contact hypersensitivity (CHS) reminiscent of ear swelling and pores and skin eosinophilia. Allergin-1-deficient mice additionally confirmed bigger numbers of CD4+ T cells and FITC-bearing DCs and better expressions of sort 2 cytokines, together with IL-5, IL-10 and IL-13, within the draining LNs than did wild sort mice. In sharp distinction, Allergin-1-deficient mice confirmed comparable degree of sort 1 CHS induced by 2,4-dinitrofluorobenzene (DNFB). These outcomes counsel that Allergin-1 on dermal DC inhibits sort 2, however not sort 1, immune responses within the sensitization section of CHS.
teitell-lab
teitell-lab

Biomolecular imaging of colorectal tumor lesions utilizing a FITC-labeled scFv-Cκ fragment antibody

For the delicate analysis of colorectal most cancers lesions, superior molecular imaging strategies utilizing cancer-specific targets have emerged. Nonetheless, points relating to the clearance of unbound probes and immunogenicity stay unresolved. To beat these limitations, we developed a small-sized scFv antibody fragment conjugated with FITC for the real-time detection of colorectal most cancers by in vivo molecular endoscopy imaging.
  • A small-sized scFv fragment can goal colon most cancers secreted protein-2 (CCSP-2), extremely expressed in colorectal adenocarcinoma tissues; furthermore, its full-length IgG probe has been used for molecular imaging beforehand. To evaluate the efficacy of anti-CCSP-2 scFv-FITC, surgical specimens have been obtained from 21 sufferers with colorectal most cancers for ex vivo molecular fluorescence evaluation, histology, and immunohistochemistry.
  • Orthotopic mice have been administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions have been noticed by real-time fluorescence colonoscopy.
  • The fluorescence imaging of human colon most cancers specimens allowed the differentiation of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression degree was discovered to be correlated with the fluorescence depth.
  • Right here, we demonstrated the feasibility and security of anti-CCSP-2 scFv-FITC for molecular imaging in addition to its potential in real-time fluorescence colonoscopy for the differential analysis of tumor lesions.

Detection of Apoptosis-like Cell Loss of life in Ustilago maydis by Annexin V-FITC Staining

Programmed cell demise (PCD) guides the transition between key developmental phases in lots of organisms. PCD additionally stays an essential destiny for a lot of organisms upon publicity to completely different stress situations. Subsequently, an perception into the development of PCD in the course of the execution of a organic phenomenon can yield important particulars of the underlying mechanism. Apoptosis, in addition to apoptosis-like programmed cell demise, constitutes one of many types of PCD in greater and decrease eukaryotes respectively.
Flipping of phosphatidylserine (PS) from the interior leaflet of the plasma membrane to the outer leaflet is among the many completely different hallmarks of apoptosis/apoptosis-like PCD that marks the initiation of the mentioned cell demise occasion. This flipping might be detected via staining of the goal cells utilizing annexin V-FITC that binds particularly to PS.
In Ustilago maydis the staining of the externally uncovered PS by annexin V-FITC is troublesome as a result of presence of cell wall. The important thing to such staining, due to this fact, depends on the light elimination of the cell wall with out considerably altering the underlying plasma membrane structure/topology. This protocol highlights the dependence of the PS staining on the extent of protoplastation of the careworn cells in Ustilago maydis.

Optimization of a strong and dependable FITC labeling course of for CE-LIF evaluation of pharmaceutical compounds utilizing design of experiments technique

 

Fluorescence, particularly laser induced fluorescence (LIF), is a robust detection method because of its specificity and excessive sensitivity. The usage of fluorescence detection hyphenated to separation method typically requires the labeling of analytes with appropriate fluorescent dye, reminiscent of FITC for the labeling of molecules presenting amino teams. However, the labeling of analytes might be a tedious, time consuming and a non-robust step of the analytical workflow.
  • On this context, the target of the current work was to suggest a strong and dependable FITC labeling course of. Major and secondary amino compounds (i.e. artificial cathinones) have been chosen as mannequin compounds as a result of they’re consultant of a big proportion of pharmaceutical small molecules.
  • Primarily based on prior data, DoE mixed with multivariate statistical modeling was carried out to optimize the method. Response time and pH of response buffer have been highlighted as essentially the most important parameters to manage the method.
  • The research confirmed additionally the good thing about brief response time to maximise the labeling effectivity. Certainly, optimum situation was outlined as response time of 32 min with ratio between FITC and analytes of 40.Four and the buffer response pH of 9.7.
  • As well as, variance element evaluation was built-in to the DoE to estimate the variability of the method and to judge its applicability for quantitative functions. These chemometric approaches helped to develop an environment friendly labeling course of in a position to attain excessive sensitivity for CE-LIF evaluation (i.e. 10 nM) with good precision (i.e. intermediate precision values decrease or shut to five %).

 

Anti-angiogenin (2A7)

YF-MA11932 100 ug
EUR 363
Description: Mouse monoclonal to angiogenin

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-Angiogenin/ANG Antibody

A00146 100ug/vial
EUR 294

Mouse Anti Human Angiogenin Monoclonal Antibody

CABT-49293MH 0.15 mg
EUR 1053

Angiogenin

PR27204 5 ug
EUR 191

Human Angiogenin (ANG)

1-CSB-RP057294h
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Angiogenin(ANG),partial expressed in E.coli

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Angiogenin Protein

20-abx261289
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Angiogenin antibody

70R-15437 100 ug
EUR 327
Description: Rabbit polyclonal Angiogenin antibody

Angiogenin Human Recombinant Protein

PROTP03950 Regular: 20ug
EUR 317
Description: ANG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 144 amino acids (25-147) and having a molecular mass of 16.4 kDa.;ANG is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Human Angiogenin (ANG) Antibody

31388-05111 150 ug
EUR 261

Human Angiogenin (ANG) Protein

20-abx166508
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Angiogenin ELISA kit

E01A0501-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

E01A0501-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

E01A0501-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

55R-2081 96 tests
EUR 712
Description: ELISA Kit for detection of Angiogenin in the research laboratory

Recombinant Angiogenin (ANG)

4-RPA007Bo01
  • EUR 504.99
  • EUR 238.00
  • EUR 1618.72
  • EUR 606.24
  • EUR 1112.48
  • EUR 401.00
  • EUR 3896.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Mu01
  • EUR 478.11
  • EUR 231.00
  • EUR 1517.92
  • EUR 572.64
  • EUR 1045.28
  • EUR 383.00
  • EUR 3644.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Mu02
  • EUR 478.11
  • EUR 231.00
  • EUR 1517.92
  • EUR 572.64
  • EUR 1045.28
  • EUR 383.00
  • EUR 3644.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Po01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Angiogenin expressed in: E.coli

Recombinant Angiogenin (ANG)

4-RPA007Ra01
  • EUR 485.28
  • EUR 233.00
  • EUR 1544.80
  • EUR 581.60
  • EUR 1063.20
  • EUR 388.00
  • EUR 3712.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Angiogenin expressed in: E.coli

Angiogenin (108-122)

HY-P1516 10mg
EUR 980

Angiogenin (108-122)

5-00673 4 x 1mg Ask for price

Angiogenin (108-123)

5-00674 4 x 1mg Ask for price

Angiogenin (ANG) Antibody

20-abx128903
  • EUR 314.00
  • EUR 133.00
  • EUR 829.00
  • EUR 439.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx129495
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx121013
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Angiogenin (ANG) Antibody

20-abx109530
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiogenin (ANG) Antibody

20-abx110971
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul