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FITC characterization of a cathepsin B-responsive nanoprobe for report of differentiation of HL60 cells into macrophages

A cathepsin B (Cat B)-responsive optical nanoprobe is designed and ready for report of HL60 differentiation into macrophage. A peptide sequence FRFK is linked to fluorescein (FITC) through the distant amino group of its lysine and N-terminated with acrylic acid (AA) to yield a molecular fluorescent probe AA-FRFK (FITC). The molecular probe is additional embedded in poly(lactic-co-glycolic acid) (PLGA) to kind a fluorescent nanoprobe AA-FRFK (FITC)@PLGA.
The resultant optical nanoprobe is degradable by lysosomal Cat B, which is expressed in macrophages with a degree of 5-10 instances of that in HL60 cells. Consequently, a big lower in fluorescence depth is related to the differentiation means of HL60 to macrophage and can be utilized as a sign of the differentiation course of.
The findings could pave a approach towards the event of a common in vitro labeling technique of exogenous stem cells for report of in vivo cell differentiation by a dual-mode imaging modality involving optical imaging and magnetic resonance imaging.

An inhibitory immunoreceptor, Allergin-1, suppresses FITC-induced sort 2 contact hypersensitivity

Though allergic contact dermatitis (ACD) is the most typical T cell-mediated inflammatory responses towards an allergen within the pores and skin, the pathogenesis of ACD stays incompletely understood. Within the sensitization section in ACD, hapten-bearing dermal dendritic cells (DCs) play a pivotal function within the transport of an antigen to the lymph nodes (LNs), the place they current the antigen to naïve T cells. Right here we report that Allergin-1, an inhibitory immunoreceptor containing immunoreceptor tyrosine-based inhibitory motif (ITIM) within the cytoplasmic area, is extremely expressed on dermal DCs.
Mice poor in Allergin-1 exhibited exacerbated fluorescein isothiocyanate (FITC)-induced sort 2 contact hypersensitivity (CHS) reminiscent of ear swelling and pores and skin eosinophilia. Allergin-1-deficient mice additionally confirmed bigger numbers of CD4+ T cells and FITC-bearing DCs and better expressions of sort 2 cytokines, together with IL-5, IL-10 and IL-13, within the draining LNs than did wild sort mice. In sharp distinction, Allergin-1-deficient mice confirmed comparable degree of sort 1 CHS induced by 2,4-dinitrofluorobenzene (DNFB). These outcomes counsel that Allergin-1 on dermal DC inhibits sort 2, however not sort 1, immune responses within the sensitization section of CHS.
teitell-lab
teitell-lab

Biomolecular imaging of colorectal tumor lesions utilizing a FITC-labeled scFv-Cκ fragment antibody

For the delicate analysis of colorectal most cancers lesions, superior molecular imaging strategies utilizing cancer-specific targets have emerged. Nonetheless, points relating to the clearance of unbound probes and immunogenicity stay unresolved. To beat these limitations, we developed a small-sized scFv antibody fragment conjugated with FITC for the real-time detection of colorectal most cancers by in vivo molecular endoscopy imaging.
  • A small-sized scFv fragment can goal colon most cancers secreted protein-2 (CCSP-2), extremely expressed in colorectal adenocarcinoma tissues; furthermore, its full-length IgG probe has been used for molecular imaging beforehand. To evaluate the efficacy of anti-CCSP-2 scFv-FITC, surgical specimens have been obtained from 21 sufferers with colorectal most cancers for ex vivo molecular fluorescence evaluation, histology, and immunohistochemistry.
  • Orthotopic mice have been administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions have been noticed by real-time fluorescence colonoscopy.
  • The fluorescence imaging of human colon most cancers specimens allowed the differentiation of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression degree was discovered to be correlated with the fluorescence depth.
  • Right here, we demonstrated the feasibility and security of anti-CCSP-2 scFv-FITC for molecular imaging in addition to its potential in real-time fluorescence colonoscopy for the differential analysis of tumor lesions.

Detection of Apoptosis-like Cell Loss of life in Ustilago maydis by Annexin V-FITC Staining

Programmed cell demise (PCD) guides the transition between key developmental phases in lots of organisms. PCD additionally stays an essential destiny for a lot of organisms upon publicity to completely different stress situations. Subsequently, an perception into the development of PCD in the course of the execution of a organic phenomenon can yield important particulars of the underlying mechanism. Apoptosis, in addition to apoptosis-like programmed cell demise, constitutes one of many types of PCD in greater and decrease eukaryotes respectively.
Flipping of phosphatidylserine (PS) from the interior leaflet of the plasma membrane to the outer leaflet is among the many completely different hallmarks of apoptosis/apoptosis-like PCD that marks the initiation of the mentioned cell demise occasion. This flipping might be detected via staining of the goal cells utilizing annexin V-FITC that binds particularly to PS.
In Ustilago maydis the staining of the externally uncovered PS by annexin V-FITC is troublesome as a result of presence of cell wall. The important thing to such staining, due to this fact, depends on the light elimination of the cell wall with out considerably altering the underlying plasma membrane structure/topology. This protocol highlights the dependence of the PS staining on the extent of protoplastation of the careworn cells in Ustilago maydis.

Optimization of a strong and dependable FITC labeling course of for CE-LIF evaluation of pharmaceutical compounds utilizing design of experiments technique

 

Fluorescence, particularly laser induced fluorescence (LIF), is a robust detection method because of its specificity and excessive sensitivity. The usage of fluorescence detection hyphenated to separation method typically requires the labeling of analytes with appropriate fluorescent dye, reminiscent of FITC for the labeling of molecules presenting amino teams. However, the labeling of analytes might be a tedious, time consuming and a non-robust step of the analytical workflow.
  • On this context, the target of the current work was to suggest a strong and dependable FITC labeling course of. Major and secondary amino compounds (i.e. artificial cathinones) have been chosen as mannequin compounds as a result of they’re consultant of a big proportion of pharmaceutical small molecules.
  • Primarily based on prior data, DoE mixed with multivariate statistical modeling was carried out to optimize the method. Response time and pH of response buffer have been highlighted as essentially the most important parameters to manage the method.
  • The research confirmed additionally the good thing about brief response time to maximise the labeling effectivity. Certainly, optimum situation was outlined as response time of 32 min with ratio between FITC and analytes of 40.Four and the buffer response pH of 9.7.
  • As well as, variance element evaluation was built-in to the DoE to estimate the variability of the method and to judge its applicability for quantitative functions. These chemometric approaches helped to develop an environment friendly labeling course of in a position to attain excessive sensitivity for CE-LIF evaluation (i.e. 10 nM) with good precision (i.e. intermediate precision values decrease or shut to five %).

 

Anti-angiogenin (2A7)

YF-MA11932 100 ug
EUR 435.6
Description: Mouse monoclonal to angiogenin

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Anti-Angiogenin/ANG Antibody

A00146 100ug/vial
EUR 352.8

Angiogenin

PR27204 5 ug
EUR 229.2

Mouse Anti Human Angiogenin Monoclonal Antibody

CABT-49293MH 0.15 mg
EUR 1263.6

Human Angiogenin (ANG)

1-CSB-RP057294h
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Angiogenin(ANG),partial expressed in E.coli

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

Angiogenin antibody

70R-15437 100 ug
EUR 392.4
Description: Rabbit polyclonal Angiogenin antibody

Angiogenin Protein

20-abx261289
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Angiogenin Antibody

DF6449 200ul
EUR 420

Angiogenin Antibody

R32581 100 ug
EUR 419

Human Angiogenin ELISA kit

55R-2081 96 tests
EUR 854.4
Description: ELISA Kit for detection of Angiogenin in the research laboratory

Human Angiogenin (ANG) Antibody

31388-05111 150 ug
EUR 313.2

Human Angiogenin (ANG) Protein

20-abx166508
  • EUR 777.60
  • EUR 326.40
  • EUR 2331.60
  • EUR 910.80
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Angiogenin ELISA kit

E01A0501-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

E01A0501-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Angiogenin ELISA kit

E01A0501-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Angiogenin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Angiogenin Human Recombinant Protein

PROTP03950 Regular: 20ug
EUR 380.4
Description: ANG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 144 amino acids (25-147) and having a molecular mass of 16.4 kDa.;ANG is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Angiogenin antibody (HRP)

60R-2121 100 ug
EUR 392.4
Description: Rabbit polyclonal Angiogenin antibody (HRP)

Angiogenin antibody (FITC)

60R-2122 100 ug
EUR 392.4
Description: Rabbit polyclonal Angiogenin antibody (FITC)

Angiogenin antibody (biotin)

60R-2123 100 ug
EUR 418.8
Description: Rabbit polyclonal Angiogenin antibody (biotin)

Angiogenin Polyclonal Antibody

42438-100ul 100ul
EUR 399.6

Angiogenin (108-122)

5-00673 4 x 1mg Ask for price

Angiogenin (108-123)

5-00674 4 x 1mg Ask for price

Angiogenin Recombinant Protein

91-183 0.05 mg
EUR 556.8
Description: Angiogenin belongs to the pancreatic ribonuclease family. Angiogenin is primarily expressed in the liver. It may act as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Angiogenin is a potent stimulator of new blood vessel formation. And Angiogenin is endocytosed and translocated to the nucleus by binding to actin on the surface of endothelial cells. Angiogenic activity is regulated by interaction with RNH1 in vivo. In addition, Angiogenin is associated with susceptibility to amyotrophic lateral sclerosis, which is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord.

Angiogenin (ANG) Antibody

20-abx171213
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

Angiogenin (ANG) Antibody

20-abx141176
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Angiogenin (ANG) Antibody

20-abx132357
  • EUR 393.60
  • EUR 994.80
  • EUR 510.00
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiogenin (ANG) Antibody

abx148162-100ug 100 ug
EUR 526.8

Angiogenin (ANG) Antibody

20-abx100298
  • EUR 493.20
  • EUR 159.60
  • EUR 1362.00
  • EUR 661.20
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug