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Development of 99mTc(CO)₃-dendrimer-FITC for cancer imaging

Examine of fluorophore and technetium labeling of poly(amido)-amine (PAMAM) era 4 (G4) dendrimer and its analysis as potential molecular imaging agent in each regular and melanoma-bearing mice, are described. Dendrimers had been first conjugated with FITC (fluorescein isothiocyanate). Dendrimer-FITC was then incubated with the intermediate [(99m)Tc(CO)(3)(H(2)O)(3)](+) and purified by gel filtration. Biodistribution and scintigraphy photos had been carried out administrating (99m)Tc(CO)(3)-dendrimer-FITC to regular mice (NM) or melanoma-bearing mice (MBM).
Cryostat tissue sections from MBM mice had been analyzed by confocal microscopy. Radiolabeling yield of dendrimer was approx. 90%. The (99m)Tc(CO)(3)-dendrimer-FITC advanced was steady for at the least 24h. Biodistribution research in NM confirmed blood clearance with hepatic and renal depuration. MBM confirmed an identical sample of biodistribution with excessive tumor uptake that allowed tumor imaging. Confocal microscopy evaluation confirmed cytoplasmic distribution of (99m)Tc(CO)(3)-dendrimer-FITC.

FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator

The efficiency of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)32+) co-doped silica particles as pH indicator was evaluated. The emission depth ratios of the pH delicate dye (FITC) and the reference dye (Ru(phen)32+) within the particles had been depending on pH of the surroundings. The modifications in emission depth ratios of the 2 dyes below totally different pH could possibly be measured below single excitation wavelength and readily visualized by bare eye below a 365-nm UV lamp.
Particularly, such FITC and Ru(phen)32+ co-doped silica particles had been recognized to indicate excessive sensitivity to pH across the pKa of FITC (6.4), making them be potential helpful as visualized pH indicator for detection of intracellular pH micro-circumstance.

PPAR gamma antibody

20R-1732 100 ug
EUR 807.6
Description: Rabbit polyclonal PPAR gamma antibody

PPAR- gamma Antibody

ABF6284 100 ug
EUR 525.6

PPAR gamma Antibody

DF6073 200ul
EUR 420

PPAR gamma Antibody

AF6284 200ul
EUR 420

PPAR gamma Antibody

AF6675 100ul
EUR 420

PPAR gamma Antibody

R30380 100 ug
EUR 419

PPAR-Gamma/ Rat PPAR- Gamma ELISA Kit

ELA-E0886r 96 Tests
EUR 1063.2

Human PPAR-gamma Antibody

11521-05011 150 ug
EUR 260.4

PPAR gamma antibody (pS112)

70R-50235 100 ul
EUR 344.4
Description: Purified Polyclonal PPAR gamma antibody

PPAR gamma antibody (Ser112)

70R-35789 100 ug
EUR 392.4
Description: Rabbit polyclonal PPAR gamma antibody (Ser112)

PPAR gamma 2 antibody

20R-PR025 100 ul
EUR 807.6
Description: Rabbit polyclonal PPAR gamma 2 antibody

Human PPAR gamma Antibody

32975-05111 150 ug
EUR 313.2

PPAR-Gamma Polyclonal Antibody

ABP52252-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112

PPAR-Gamma Polyclonal Antibody

ABP52252-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112

PPAR-Gamma Polyclonal Antibody

ABP52252-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112

PPAR-Gamma Polyclonal Antibody

ABP57258-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

PPAR-Gamma Polyclonal Antibody

ABP57258-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

PPAR-Gamma Polyclonal Antibody

ABP57258-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

Anti-PPAR gamma Antibody

A1712-100
EUR 405.6

Anti-PPAR gamma Antibody

A1712-30T
EUR 175.2

Polyclonal PPAR gamma Antibody

AMR09445G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPAR gamma . This antibody is tested and proven to work in the following applications:

Anti-PPAR gamma antibody

PAab06660 100 ug
EUR 426

Anti-PPAR gamma antibody

PAab06661 100 ug
EUR 426

anti- PPAR gamma antibody

FNab06660 100µg
EUR 606.3
Description: Antibody raised against PPAR gamma

anti- PPAR gamma antibody

FNab06661 100µg
EUR 606.3
Description: Antibody raised against PPAR gamma

Anti-PPAR-gamma antibody

STJ98330 100 µl
EUR 280.8
Description: Mouse monoclonal to PPAR-gamma.

Anti-PPAR-gamma antibody

STJ98538 100 µl
EUR 280.8
Description: Mouse monoclonal to PPAR-gamma.

Anti-PPAR-gamma antibody

STJ95201 200 µl
EUR 236.4
Description: PPAR-gamma is a protein encoded by the PPARG gene which is approximately 57,6 kDa. PPAR-gamma is localised to the cytoplasm and nucleus. It is involved in regulation of lipid metabolism, gene expression, the nuclear receptor transcription pathway and AMP-activated protein kinase signalling. This protein falls under the peroxisome proliferator-activated receptor subfamily. It is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once logand activated, the nuclear receptor binds to DNA specific PPAR response elements and modulates the transcription of its target genes, such as acyl-CoA oxidase. PPAR-gamma has the highest expression in adipose tissue. Mutations in the PPARG gene may result in diabetes mellitus and is a cardiovascular disease risk factor. STJ95201 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PPAR-gamma protein.

Anti-PPAR-gamma antibody

STJ97460 200 µl
EUR 236.4
Description: PPAR-gamma is a protein encoded by the PPARG gene which is approximately 57,6 kDa. PPAR-gamma is localised to the nucleus and cytoplasm. It is involved in gene expression, metabolism, nuclear receptor transcription pathway and AMP-activated protein kinase signalling. This protein falls under the peroxisome proliferator-activated receptor family. It forms heterodimers with retinoid X receptors and these heterodimers regulate transcription of various genes. It is also a regulator of adipocyte differentiation. PPAR-gamma is expressed highly in adipose tissue. Mutations in the PPARG gene may result in obesity and lipodystrophy. STJ97460 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous PPAR-gamma protein.

PPAR gamma Polyclonal Antibody

A71563
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

PPAR gamma Polyclonal Antibody

A71564
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

PPAR gamma Polyclonal Antibody

A71565
  • Ask for price
  • Ask for price
  • 50 ul
  • 100 ul

PPAR gamma Polyclonal Antibody

A71563-050 50 ul
EUR 302.5

PPAR gamma Polyclonal Antibody

A71563-100 100 ul
EUR 423.5

PPAR gamma Polyclonal Antibody

A71564-050 50 ul
EUR 302.5

PPAR gamma Polyclonal Antibody

A71564-100 100 ul
EUR 423.5

PPAR gamma Polyclonal Antibody

A71565-050 50 ul Ask for price

PPAR gamma Polyclonal Antibody

A71565-100 100 ul Ask for price

PPAR gamma Antibody / PPARG

F54004-0.1ML 0.1 ml
EUR 379