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Development of 99mTc(CO)₃-dendrimer-FITC for cancer imaging

Examine of fluorophore and technetium labeling of poly(amido)-amine (PAMAM) era 4 (G4) dendrimer and its analysis as potential molecular imaging agent in each regular and melanoma-bearing mice, are described. Dendrimers had been first conjugated with FITC (fluorescein isothiocyanate). Dendrimer-FITC was then incubated with the intermediate [(99m)Tc(CO)(3)(H(2)O)(3)](+) and purified by gel filtration. Biodistribution and scintigraphy photos had been carried out administrating (99m)Tc(CO)(3)-dendrimer-FITC to regular mice (NM) or melanoma-bearing mice (MBM).
Cryostat tissue sections from MBM mice had been analyzed by confocal microscopy. Radiolabeling yield of dendrimer was approx. 90%. The (99m)Tc(CO)(3)-dendrimer-FITC advanced was steady for at the least 24h. Biodistribution research in NM confirmed blood clearance with hepatic and renal depuration. MBM confirmed an identical sample of biodistribution with excessive tumor uptake that allowed tumor imaging. Confocal microscopy evaluation confirmed cytoplasmic distribution of (99m)Tc(CO)(3)-dendrimer-FITC.

FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator

The efficiency of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)32+) co-doped silica particles as pH indicator was evaluated. The emission depth ratios of the pH delicate dye (FITC) and the reference dye (Ru(phen)32+) within the particles had been depending on pH of the surroundings. The modifications in emission depth ratios of the 2 dyes below totally different pH could possibly be measured below single excitation wavelength and readily visualized by bare eye below a 365-nm UV lamp.
Particularly, such FITC and Ru(phen)32+ co-doped silica particles had been recognized to indicate excessive sensitivity to pH across the pKa of FITC (6.4), making them be potential helpful as visualized pH indicator for detection of intracellular pH micro-circumstance.

PPAR gamma Antibody

3809-30T
EUR 146

PPAR gamma Antibody

49371-100ul 100ul
EUR 333

PPAR gamma Antibody

49371-50ul 50ul
EUR 239

PPAR gamma antibody

70R-35496 100 ug
EUR 327
Description: Purified Rabbit polyclonal PPAR gamma antibody

PPAR-gamma Antibody

AF6284 200ul
EUR 304
Description: PPAR-gamma Antibody detects endogenous levels of total PPAR-gamma.

PPAR- gamma Antibody

ABF6284 100 ug
EUR 438

PPAR-Gamma/ Rat PPAR- Gamma ELISA Kit

ELA-E0886r 96 Tests
EUR 886

PPAR gamma 2 antibody

20R-PR025 100 ul
EUR 673
Description: Rabbit polyclonal PPAR gamma 2 antibody

Human PPAR gamma Antibody

32975-05111 150 ug
EUR 261

Human PPAR-gamma Antibody

11521-05011 150 ug
EUR 217

Polyclonal PPAR gamma Antibody

AMR09445G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPAR gamma . This antibody is tested and proven to work in the following applications:

PPAR gamma antibody (Ser112)

70R-35789 100 ug
EUR 327
Description: Rabbit polyclonal PPAR gamma antibody (Ser112)

PPAR gamma antibody (pS112)

70R-50235 100 ul
EUR 287
Description: Purified Polyclonal PPAR gamma antibody

PPAR-Gamma Polyclonal Antibody

ABP52252-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112
  • Applications tips:
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112

PPAR-Gamma Polyclonal Antibody

ABP52252-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112
  • Applications tips:
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112

PPAR-Gamma Polyclonal Antibody

ABP52252-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112
  • Applications tips:
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PPAR-? around the non-phosphorylation site of S112

Anti-PPAR gamma Antibody

A1712-100
EUR 338

Anti-PPAR gamma Antibody

A1712-30T
EUR 146

PPAR-Gamma Polyclonal Antibody

ABP57258-003ml 0.03ml
EUR 158
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

PPAR-Gamma Polyclonal Antibody

ABP57258-01ml 0.1ml
EUR 289
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

PPAR-Gamma Polyclonal Antibody

ABP57258-02ml 0.2ml
EUR 414
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A polyclonal antibody for detection of PPAR-? from Human, Mouse, Rat. This PPAR-? antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein

anti- PPAR gamma antibody

FNab06660 100µg
EUR 505.25
  • Recommended dilution: WB: 1:500-1:2000
  • IHC: 1:50-1:200
  • IF: 1:10-1:100
  • IP: 1:500-1:1000
  • Immunogen: peroxisome proliferator-activated receptor gamma
  • Uniprot ID: P37231
  • Gene ID: 5468
  • Research Area: Signal Transduction, Metabolism, Cardiovascular, Dev
  • Show more
Description: Antibody raised against PPAR gamma

anti- PPAR gamma antibody

FNab06661 100µg
EUR 505.25
  • Immunogen: peroxisome proliferator-activated receptor gamma
  • Uniprot ID: P37231
  • Gene ID: 5468
  • Research Area: Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells
Description: Antibody raised against PPAR gamma

Anti-PPAR gamma antibody

PAab06660 100 ug
EUR 355

Anti-PPAR gamma antibody

PAab06661 100 ug
EUR 355

Anti-PPAR-gamma antibody

STJ95201 200 µl
EUR 197
Description: PPAR-gamma is a protein encoded by the PPARG gene which is approximately 57,6 kDa. PPAR-gamma is localised to the cytoplasm and nucleus. It is involved in regulation of lipid metabolism, gene expression, the nuclear receptor transcription pathway and AMP-activated protein kinase signalling. This protein falls under the peroxisome proliferator-activated receptor subfamily. It is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once logand activated, the nuclear receptor binds to DNA specific PPAR response elements and modulates the transcription of its target genes, such as acyl-CoA oxidase. PPAR-gamma has the highest expression in adipose tissue. Mutations in the PPARG gene may result in diabetes mellitus and is a cardiovascular disease risk factor. STJ95201 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PPAR-gamma protein.

Anti-PPAR-gamma antibody

STJ97460 200 µl
EUR 197
Description: PPAR-gamma is a protein encoded by the PPARG gene which is approximately 57,6 kDa. PPAR-gamma is localised to the nucleus and cytoplasm. It is involved in gene expression, metabolism, nuclear receptor transcription pathway and AMP-activated protein kinase signalling. This protein falls under the peroxisome proliferator-activated receptor family. It forms heterodimers with retinoid X receptors and these heterodimers regulate transcription of various genes. It is also a regulator of adipocyte differentiation. PPAR-gamma is expressed highly in adipose tissue. Mutations in the PPARG gene may result in obesity and lipodystrophy. STJ97460 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous PPAR-gamma protein.

Anti-PPAR-gamma antibody

STJ98330 100 µl
EUR 234
Description: Mouse monoclonal to PPAR-gamma.

Anti-PPAR-gamma antibody

STJ98538 100 µl
EUR 234
Description: Mouse monoclonal to PPAR-gamma.

PPAR gamma protein

E20-80087 20ug
EUR 408

anti-PPAR gamma

YF-PA24433 50 ul
EUR 334
Description: Mouse polyclonal to PPAR gamma

Anti-PPAR gamma/PPARG Antibody

A00449-2 100ug/vial
EUR 294

Phospho-PPAR gamma (Ser112) Antibody

AF3284 200ul
EUR 304
Description: Phospho-PPAR- gamma (Ser112) Antibody detects endogenous levels of PPAR- gamma only when phosphorylated at Serine 112.

Phospho- PPAR- gamma (Ser112) Antibody

ABF3284 100 ug
EUR 438

Anti-PPAR gamma/PPARG Antibody

PA1320 100ug/vial
EUR 294

Antibody for Human PPAR gamma

SPC-1308D 0.1ml
EUR 314
  • Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclea
  • Show more
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is unconjugated.

Antibody for Human PPAR gamma

SPC-1308D-A390 0.1ml
EUR 361
  • Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclea
  • Show more
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 390.

Antibody for Human PPAR gamma

SPC-1308D-A488 0.1ml
EUR 360
  • Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclea
  • Show more
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 488.

Antibody for Human PPAR gamma

SPC-1308D-A565 0.1ml
EUR 360
  • Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclea
  • Show more
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 565.