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Development of 99mTc(CO)₃-dendrimer-FITC for cancer imaging

Examine of fluorophore and technetium labeling of poly(amido)-amine (PAMAM) era 4 (G4) dendrimer and its analysis as potential molecular imaging agent in each regular and melanoma-bearing mice, are described. Dendrimers had been first conjugated with FITC (fluorescein isothiocyanate). Dendrimer-FITC was then incubated with the intermediate [(99m)Tc(CO)(3)(H(2)O)(3)](+) and purified by gel filtration. Biodistribution and scintigraphy photos had been carried out administrating (99m)Tc(CO)(3)-dendrimer-FITC to regular mice (NM) or melanoma-bearing mice (MBM).
Cryostat tissue sections from MBM mice had been analyzed by confocal microscopy. Radiolabeling yield of dendrimer was approx. 90%. The (99m)Tc(CO)(3)-dendrimer-FITC advanced was steady for at the least 24h. Biodistribution research in NM confirmed blood clearance with hepatic and renal depuration. MBM confirmed an identical sample of biodistribution with excessive tumor uptake that allowed tumor imaging. Confocal microscopy evaluation confirmed cytoplasmic distribution of (99m)Tc(CO)(3)-dendrimer-FITC.

FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator

The efficiency of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)32+) co-doped silica particles as pH indicator was evaluated. The emission depth ratios of the pH delicate dye (FITC) and the reference dye (Ru(phen)32+) within the particles had been depending on pH of the surroundings. The modifications in emission depth ratios of the 2 dyes below totally different pH could possibly be measured below single excitation wavelength and readily visualized by bare eye below a 365-nm UV lamp.
Particularly, such FITC and Ru(phen)32+ co-doped silica particles had been recognized to indicate excessive sensitivity to pH across the pKa of FITC (6.4), making them be potential helpful as visualized pH indicator for detection of intracellular pH micro-circumstance.

PPAR gamma antibody

70R-35496 100 ug
EUR 392.4
Description: Purified Rabbit polyclonal PPAR gamma antibody

PPAR- gamma Antibody

ABF6284 100 ug
EUR 525.6

PPAR gamma Antibody

DF6073 200ul
EUR 420

PPAR gamma Antibody

R30380 100 ug
EUR 356.15
Description: The peroxisome proliferator-activated receptors are a group of three nuclear receptor isoforms, PPAR gamma, alpha, delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. Isoforms 1 and 2 result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al.(2003) found that it is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases its expression in endothelial cells and that loss of expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.

PPAR gamma Antibody

E38PA4778 100ul
EUR 225
Description: Available in various conjugation types.

PPAR gamma Antibody

AF6284-100ul 100ul
EUR 280

PPAR gamma Antibody

AF6284-200ul 200ul
EUR 350

PPAR gamma Antibody

AF6675-100ul 100ul
EUR 280

PPAR gamma Antibody

AF6675-200ul 200ul
EUR 350

PPAR gamma Antibody

AF6284 100ul
EUR 280
Description: Human,Mouse,Rat

PPAR gamma Antibody

AF6675 100ul
EUR 280
Description: Human,Mouse,Rat

PPAR gamma Antibody

49371 100ul
EUR 499

PPAR gamma Antibody / PPARG

F54004-0.1ML 0.1 ml
EUR 322.15
Description: Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, suc

PPAR gamma Antibody / PPARG

RQ6963 100 ug
EUR 356.15
Description: Peroxisome proliferator- activated receptor gamma (PPAR-gamma or PPARG), also known as the glitazone reverse insulin resistance receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3) is a type II nuclear receptor (protein regulating genes) that in humans is encoded by the PPARG gene. This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

PPAR gamma 2 antibody

20R-PR025 100 ul
EUR 807.6
Description: Rabbit polyclonal PPAR gamma 2 antibody

anti- PPAR gamma antibody

FNab06660 100µg
EUR 606.3
Description: Antibody raised against PPAR gamma

anti- PPAR gamma antibody

FNab06661 100µg
EUR 606.3
Description: Antibody raised against PPAR gamma

PPAR gamma antibody (pS112)

70R-50235 100 ul
EUR 344.4
Description: Purified Polyclonal PPAR gamma antibody

Human PPAR gamma Antibody

32975-05111 150 ug
EUR 313.2

PPAR gamma antibody (Ser112)

70R-35789 100 ug
EUR 392.4
Description: Rabbit polyclonal PPAR gamma antibody (Ser112)

Polyclonal PPAR gamma Antibody

AMR09445G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPAR gamma . This antibody is tested and proven to work in the following applications:

PPAR gamma (pS112) Antibody

E38PA1916 100ul
EUR 225
Description: Available in various conjugation types.

PPAR gamma Polyclonal Antibody

A71563
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

PPAR gamma Polyclonal Antibody

A71564
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

PPAR gamma Polyclonal Antibody

A71565
  • Ask for price
  • Ask for price
  • 50 ul
  • 100 ul

OASL01039-100UL - PPAR gamma Antibody

OASL01039-100UL 100ul
EUR 269

Antibody for Human PPAR gamma

SPC-1308D 0.1ml
EUR 376.8
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is unconjugated.

Antibody for Human PPAR gamma

SPC-1308D-A390 0.1ml
EUR 433.2
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 390.

Antibody for Human PPAR gamma

SPC-1308D-A488 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 488.

Antibody for Human PPAR gamma

SPC-1308D-A565 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 565.

Antibody for Human PPAR gamma

SPC-1308D-A594 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 594.

Antibody for Human PPAR gamma

SPC-1308D-A633 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 633.

Antibody for Human PPAR gamma

SPC-1308D-A655 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 655.

Antibody for Human PPAR gamma

SPC-1308D-A680 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 680.

Antibody for Human PPAR gamma

SPC-1308D-A700 0.1ml
EUR 432
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to ATTO 700.

Antibody for Human PPAR gamma

SPC-1308D-ALP 0.1ml
EUR 426
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to Alkaline Phosphatase.

Antibody for Human PPAR gamma

SPC-1308D-APC 0.1ml
EUR 430.8
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to APC .

Antibody for Human PPAR gamma

SPC-1308D-APCCY7 0.1ml
EUR 518.4
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to APC/Cy7.

Antibody for Human PPAR gamma

SPC-1308D-BI 0.1ml
EUR 428.4
Description: A polyclonal antibody for PPAR gamma from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with Human A synthesized peptide derived from human PPAR-gamma. The Antibody is tested and validated for WB, IHC assays with the following recommended dilutions: WB (1:2000), IHC (1:200). This PPAR gamma antibody is conjugated to Biotin.
Frank Rivera