Instantly label your major antibody with HRP

​​​Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which could be conjugated to antibodies and proteins to be used in a wide range of purposes.

The enzyme label could be visualized via chromogenic reactions. For instance, diaminobenzidine (DAB) within the presence of hydrogen peroxide (H₂0₂) is transformed right into a water-insoluble brown pigment. Different substrates that can be utilized to measure horseradish peroxidase exercise embrace ABTS, TMB, and TMBUS.

HRP is a well-liked detection label utilized in analysis. Antibody-HRP conjugates are generally utilized in ELISA, IHC, and western blotting. HRP could be conjugated to the first antibody for direct detection or secondary antibody for oblique detection.

Direct detection is usually a most well-liked methodology inside these purposes to keep away from cross-species reactivity and to eradicate extra laborious wash and separation steps, particularly in time-consuming protocols. Nonetheless, instantly conjugating HRP to the antibody or protein of selection could be tough and labor-intensive if utilizing conventional methodology.

Our Lightning-Hyperlink HRP antibody labeling equipment permits the direct conjugation of your antibody, protein, or peptide to HRP to be used in any software. The equipment solely requires 30 seconds of hands-on time, and conjugates are prepared to make use of after solely three hours. Moreover, there isn’t a want for additional wash or separation steps submit conjugation.