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Peripheral blood T lymphocytosis in thymoma: an insight into immunobiology

Objective: Peripheral blood T lymphocytosis (PBTL) is a uncommon, but poorly understood manifestations of thymoma, which is postulated to be linked with autoimmune/paraneoplastic manifestations resembling myasthenia gravis (MG), pure purple cell aplasia (PRCA), and so on.; extra generally encountered on this neoplasm.
Methodology: We goal to explain the flowcytometric immunophenotypic knowledge of PBTL in a 43-year-old male; 6 months after profitable completion of chemoradiotherapy (CT/RT) for a big, invasive, and metastatic sort B1 thymoma; and current a complete assessment of all such instances reported over final 42 years (N = 21) (1977-2019).
Outcome: A bigger dimension of the tumors (≥ 10 cm), presence of native invasion and/or distant metastasis, and sort B (cortical or lymphocyte wealthy) histology had been extra prone to be related to PBTL. Tumors related to MG/PRCA (N = 9/21) are likely to have decrease PBTL in comparison with these with out such manifestations; and PBTL subsided following thymectomy with or with out CT/RT. Immunophenotypic evaluation of PB revealed a CD8 + > CD4 + mature (naïve) polyclonal T cells resembling late cortical thymocytes.
Conclusion: Thymic intratumoral microenvironment would possibly affect prevalence PBTL which will have a pathophysiologic hyperlink to improvement of autoimmune manifestations. Immunophenotypic traits of peripheral blood lymphoid cells ought to be the clue for correct characterization and to keep away from a misdiagnosis of a lymphoproliferative neoplasm.

Immunobiology and pathogenesis of hepatitis B virus an infection

Hepatitis B virus (HBV) is a non-cytopathic, hepatotropic virus with the potential to trigger a persistent an infection, in the end resulting in cirrhosis and hepatocellular carcinoma.

Over the previous 4 many years, the essential ideas of HBV gene expression and replication in addition to the viral and host determinants governing an infection final result have been largely uncovered. Whereas HBV seems to induce little or no innate immune activation, the adaptive immune response mediates each viral clearance in addition to liver illness.

Right here, we assessment our present information on the immunobiology and pathogenesis of HBV an infection, focusing specifically on the position of CD8+ T cells and on a number of latest breakthroughs that problem present dogmas.

For instance, we now belief that HBV integration into the host genome usually serves as a related supply of hepatitis B floor antigen (HBsAg) expression throughout power an infection, presumably triggering dysfunctional T cell responses and favouring detrimental immunopathology.

Additional, the distinctive haemodynamics and anatomy of the liver – and the adjustments they ceaselessly endure throughout illness development to liver fibrosis and cirrhosis – profoundly affect T cell priming, differentiation and performance.

We additionally focus on why therapeutic approaches that restrict the intrahepatic inflammatory processes triggered by HBV-specific T cells is likely to be surprisingly useful for sufferers with power an infection.

 

Schistosomes within the Lung: Immunobiology and Alternative

Schistosome an infection is a significant trigger of worldwide morbidity, notably in sub-Saharan Africa. Nevertheless, there is no such thing as a efficient vaccine for this main uncared for tropical illness, and re-infection routinely happens after chemotherapeutic therapy. Following invasion by the pores and skin, larval schistosomula enter the circulatory system and migrate by the lung earlier than maturing to maturity within the mesenteric or urogenital vasculature.

 

Eggs launched from grownup worms can turn out to be trapped in numerous tissues, with resultant inflammatory responses resulting in hepato-splenic, intestinal, or urogenital illness – processes which were extensively studied in recent times.

 

In distinction, though lung pathology can happen in each the acute and power phases of schistosomiasis, the mechanisms underlying pulmonary illness are notably poorly understood. In power an infection, egg-mediated fibrosis and vascular destruction can result in the formation of portosystemic shunts by which eggs can embolise to the lungs, the place they’ll set off granulomatous illness.

 

Acute schistosomiasis, or Katayama syndrome, which is primarily evident in non-endemic people, happens throughout pulmonary larval migration, maturation, and preliminary egg-production, usually involving fever and a cough with an accompanying immune cell infiltrate into the lung. Importantly, lung migrating larvae will not be only a reason behind irritation and pathology however are a key goal for future vaccine design.

 

Nevertheless, vaccine efforts are hindered by a restricted understanding of what constitutes a protecting immune response to larvae. On this assessment, we discover the present understanding of pulmonary immune responses and inflammatory pathology in schistosomiasis, highlighting necessary unanswered questions and areas for future analysis.

teitell-lab
teitell-lab

Immunobiology and nanotherapeutics of extreme acute respiratory syndrome 2 (SARS-CoV-2): a present replace

The emergence of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) constitutes probably the most important world public well being problem in a century. It has reignited analysis curiosity in coronavirus.

 

  • Whereas little data is out there, analysis is at present in progress to comprehensively perceive the final biology and immune response mechanism towards SARS-CoV-2. The spike proteins (S protein) of SARS-CoV-2 carry out an important operate in viral an infection institution. ACE2 and TMPRSS2 play a pivotal position in viral entry. “
  • Upon viral entry, the launched pro-inflammatory proteins (cytokines and chemokines) trigger the migration of the T cells, monocytes, and macrophages to the an infection website.
  • IFNϒ launched by T cells initiates a loop of pro-inflammatory suggestions. The inflammatory state might additional improve with a rise in immune dysfunction liable for the an infection’s development.
  • A therapy strategy that forestalls ACE2-mediated viral entry and reduces inflammatory response is an important therapeutic intervention technique, and nanomaterials and their conjugates are promising candidates. Nanoparticles can inhibit viral entry and replication.
  • Nanomaterials have additionally discovered software in focused drug supply and in addition in growing a vaccine towards SARS-CoV-2. Right here, we briefly summarize the origin, transmission, and medical options of SARS-CoV-2. We then mentioned the immune response mechanisms of SARS-CoV-2.
  • Lastly, we additional mentioned nanotechnology’s potentials as an intervention technique towards SARS-CoV-2 an infection. All these understandings shall be essential in growing therapeutic methods towards SARS-CoV-2.

PD-1 immunobiology in glomerulonephritis and renal cell carcinoma

Background: Programmed cell loss of life protein (PD)-1 receptors and ligands on immune cells and kidney parenchymal cells assist preserve immunological homeostasis within the kidney. Dysregulated PD-1:PD-L1 binding interactions happen through the pathogenesis of glomerulopathies and renal cell carcinoma (RCC). The regulation of those molecules within the kidney is necessary to PD-1/PD-L1 immunotherapies that deal with RCC and should induce glomerulopathies as an hostile occasion.

Strategies: The expression and performance of PD-1 molecules on immune and kidney parenchymal cells had been reviewed within the wholesome kidney, PD-1 immunotherapy-induced nephrotoxicity, glomerulopathies and RCC.

Outcomes: PD-1 and/or its ligands are expressed on kidney macrophages, dendritic cells, lymphocytes, and renal proximal tubule epithelial cells. Vitamin D3, glutathione and AMP-activated protein kinase (AMPK) regulate hypoxic cell indicators concerned within the expression and performance of PD-1 molecules.

These pathways are altered in kidney illness and are linked to the manufacturing of vascular endothelial development issue, erythropoietin, adiponectin, interleukin (IL)-18, IL-23, and chemokines that bind CXCR3, CXCR4, and/or CXCR7.

These components are differentially produced in glomerulonephritis and RCC and could also be necessary biomarkers in sufferers that obtain PD-1 therapies and/or develop glomerulonephritis as an hostile occasion CONCLUSION: By evaluating the features of the PD-1 axis in glomerulopathies and RCC, we recognized related chemokines concerned within the recruitment of immune cells and distinct mediators in T cell differentiation.

 

The expression and performance of PD-1 and PD-1 ligands in diseased tissue and notably on double-negative T cells and parenchymal kidney cells wants continued exploration. The doable regulation of the PD-1 axis by vitamin D3, glutathione and/or AMPK cell indicators could also be necessary to kidney illness and the PD-1 immunotherapeutic response.

293AAV Cell Line

AAV-100 1 vial
EUR 405

293LTV Cell Line

LTV-100 1 vial
EUR 405

293RTV Cell Line

RV-100 1 vial
EUR 405

MCF7 [MCF-7] Cell Line

CL-0149 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

293/GFP Cell Line

AKR-200 1 vial
EUR 460

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 460

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 460

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 460

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 460

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 460

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 460

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

MCF-7-LUC cells

S0006002 One Frozen vial
EUR 420

PKCa Stable Expressing MCF-7 (MCF-7/PKCa20) Cell Line

T6115 1x10^6 cells / 1.0 ml
EUR 3950

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 770

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 770

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 770

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

MCF 10A Cell Line

CL-0525 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

cDNA - Human Tumor Cell Line: MCF 7

C1255830 40 reactions
EUR 424

Flavopiridol-Resistant MCF-7 Cell Line (FLV100)

T8033 1x10^6 cells / 1.0 ml
EUR 2950

Flavopiridol-Resistant MCF-7 Cell Line (FLV1000)

T8035 1x10^6 cells / 1.0 ml Ask for price

Luc-Akt-PH Stable MCF7 Cell Line

T3160 1x10^6 cells / 1.0 ml
EUR 3950

Total RNA - Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 229

Genomic DNA - Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 282

Total Protein - Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 216

Membrane Protein - Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 311

Exosome derived from human breast cancer, noninvasive cell line (MCF-7 cell line)

Exo-CH06 Each
EUR 1249.5
Description: Exosome

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 262

AAV2-Luc Control Virus

AAV-320 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 2.

AAV1-Luc Control Virus

AAV-321 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 1.

AAV3-Luc Control Virus

AAV-323 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 3.

AAV4-Luc Control Virus

AAV-324 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 4.

AAV5-Luc Control Virus

AAV-325 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 5.

AAV6-Luc Control Virus

AAV-326 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 6.

Tissue, Section, Human Disease, Human Tumor Cell Line, MCF-7 (Paraffin)

MBS640859-5Slides 5Slides
EUR 510

Tissue, Section, Human Disease, Human Tumor Cell Line, MCF-7 (Paraffin)

MBS640859-5x5Slides 5x5Slides
EUR 2145

GAS(Luc) Report Cell Line-HeLa

ABC-RC099D 1 vial Ask for price
Description: The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of IFN gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the IFN gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind IFN gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.

GAL4 Report Cell Line(Luc)-HEK293

ABC-RC113D 1 vial Ask for price
Description: The GAL4 Reporter (Luc) andndash; HEK293 Cell Line contains a firefly luciferase gene under the control of a multimerized GAL4 upstream activation sequence (UAS) stably integrated into HEK293 cells. The cell line does not contain any exogenous activators of the GAL4 reporter.

NFAT Reporter (Luc) - Jurkat Cell Line

60621 2 vials
EUR 2295
Description: The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the_x000D_NFAT response element stably integrated into Jurkat cells. This cell line has been validated for_x000D_response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as_x000D_a control cell line for other NFAT reporter cell lines expressing various immune checkpoint_x000D_receptors.

GAL4 Reporter (Luc)-HEK293 Cell Line

60656 2 vials
EUR 1095
Description: The GAL4 Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of a multimerized GAL4 upstream activation sequence (UAS) stably integrated into HEK293 cells. The cell line does not contain any exogenous activators of the GAL4 reporter and can be used alongside BPS Cat. #60655 as a control.

Foxp3(Luc) Report Cell Line-Jurkat

ABC-RC106D 1 vial Ask for price
Description: Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 409

STAT5 Reporter (Luc) - Ba/F3 Cell line

79772 2 vials
EUR 2275
Description: The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 715

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3095

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 750

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3215

IRF Reporter (Luc) - THP-1 Cell line

79858 2 vials
EUR 1810
Description: The Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in human monocytic cell line THP-1. It contains a firefly luciferase gene driven by multimerized ISRE (Interferon Stimulated Response Element) located upstream of the minimal TATA promoter. _x000D_The cGAS-STING pathway acts to detect cytosolic DNA and induce an immune response. Briefly, upon binding DNA, the protein cGAS (cyclic GMP-AMP Synthase) triggers reaction of GTP and ATP to form cGAMP. cGAMP binds to STING (Stimulator of Interferon Genes) which triggers phosphorylation of IRF3 via TBK1. IRF3 can then bind to interferon-stimulated responsive elements (ISRE) in the nucleus and leads to IFN-α/β production. The IRF reporter (Luc)-THP-1 cell line is highly responsive to STING and CDS ligands.

GR-GAL4 Report Cell Line(Luc)-HEK293

ABC-RC114D 1 vial Ask for price
Description: The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of DXMS and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

NF-κB Reporter (Luc) - HCT116 Cell Line

60623 2 vials
EUR 1095
Description: NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).

NF-κB reporter (Luc) - HEK293 Cell line

60650 2 vials
EUR 1365
Description: The NF-κB reporter (Luc) HEK293 cell line is designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.

NF-kB reporter (Luc) - HEK293 Cell line

GWB-PS76F8 2X10(6)cells Ask for price

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Tissue, Total Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)

MBS657022-1mg 1mg
EUR 565

Tissue, Total Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)

MBS657022-5x1mg 5x1mg
EUR 2315

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Tissue, Membrane Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)

MBS639777-01mg 0.1mg
EUR 570

Tissue, Membrane Protein, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima)

MBS639777-5x01mg 5x0.1mg
EUR 2345

STAT3 Reporter (Luc) - HEK293 Cell line (Puromycin)

79800-P 2 vials
EUR 3730
Description: The STAT3 Reporter (Luc)-HEK293 cell line is designed for monitoring STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

Luciferase Stable 17L3C (17L3C-luc) Cell Line

T6240 1x10^6 cells / 1.0 ml
EUR 3950

Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line

60628 2 vials
EUR 7645
Description: Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.

NF- κB Reporter (Luc) - Raw 264.7 Cell line

79978 2 vials
EUR 2045
Description: The NF-κB reporter (Luc)-Raw 264.7 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 420

PAI-1 Report Cell Line (Luc)-Mv1 Lu

ABC-RC199D 1 vial Ask for price
Description: PAI-1 Reporter (Luc)-Mv1 Lu cell line is designed for monitoring transforming growth factor β (TGF-β)-induced plasminogen activator inhibitor-1 (PAI-1) expression. Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression.PAI-1 Reporter (Luc) –Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity.

NF- κB Reporter (Luc) - THP-1 Cell Line

79645 2 vials
EUR 1900
Description: The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

PAI-1 Reporter (Luc) - Mv1 Lu Cell Line

60544 2 vials
EUR 3595
Description: PAI-1 Reporter (Luc)-Mv1 Lu cell line is designed for monitoring transforming growth factor β (TGF-β)-induced plasminogen activator inhibitor-1 (PAI-1) expression. Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression._x000D__x000D_PAI-1 Reporter (Luc) -Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity. _x000D_

NF-κB reporter (Luc) - NIH/3T3 Cell line

79469 2 vials
EUR 1900
Description: The NF-κB reporter (Luc)-NIH/3T3 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect™ MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 320

NF-κB Reporter (Luc) - CHO-K1 Cell Line

60622 2 vials
EUR 1095
Description: An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κB transcription factor through luminescence readout.). This cell line responds to human cytokine IL-1β, responds moderately to human TNF, and does not respond to human IFN-λ (2 µg/ml). Reducing the amount of serum during incubation period may increase the sensitivity to cytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell line provides an excellent platform to enable exogenous expression of a protein of interest to study its downstream effect on NF-κB signaling.

CD40/NF-κB Report Cell Line(Luc) - HEK293

ABC-RC111D 1 vial Ask for price
Description: Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by real-time qPCR and Western Blot. This NF-ĸB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-andkappa;B response element located upstream of the TATA promoter. Following activation by human CD40 ligand, NF-ĸB transcription factor binds to the DNA response elements to induce transcription of the luciferase gene.

Radius™ 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 425

Radius™ 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 1820

Radius™ 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 495

Radius™ 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2095

Radius™ 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 445

Radius™ 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 1895

NF-κB Reporter (Luc) - A549 Stable Cell Line

60625 2 vials
EUR 1915
Description: NF-κB luciferase reporter construct is stably integrated into the genome of A549 cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

STAT5 Reporter (Luc)- U937 Cell Line (GM-CSF)

79941 2 vials
EUR 1980
Description: The STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by GM-CSF, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect™ BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 455

CytoSelect™ 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 640

CytoSelect™ 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 2695

CytoSelect™ Cell Viability and Cytotoxicity Assay

CBA-240 96 assays
EUR 305

Tissue, Total RNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinoma), BioGenomics

MBS638573-005mg 0.05mg
EUR 485

Tissue, Total RNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinoma), BioGenomics

MBS638573-5x005mg 5x0.05mg
EUR 1960

CytoSelect 24-well Laminin Cell Invasion, Colorimetric

CBA-110-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect™ 24-Well Cell Co-Culture System

CBA-160 24 assays
EUR 365

CytoSelect™ 24-Well Cell Co-Culture System

CBA-160-5 5 x 24 assays
EUR 1585

CytoSelect™ 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 575

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 320

Tissue, Genomic DNA, Human Tumor Cell Line, MCF 7 (Human Breast Adenocarcinima), BioGenomics

MBS654437-01mg 0.1mg
EUR 505
Frank Rivera