Four Which equipment ought to I take advantage of for my urine and plasma samples?
A: Use DiagExo® equipment (Cat.#: P120) to isolate exosome from urine and Use PureExo® equipment for serum (Cat.#: P101) to isolate exosome from serum.

5, Can I take advantage of your equipment to isolate exosome or endothelial microparticles from cultured cells and from plasma?
A: Our P100 equipment can be utilized to isolate exosome from both cell tradition medium or serum. It’s not for cells.

6 Is your exosome prep contaminated with IgG and/or beads?
A: We use particular reagents to seize exosomes. We didn’t use antibody or beads for the isolation. So the ultimate exosome prep is just not contaminated with synthetic IgG or beads.

7, Do your exosome prep accommodates Albumin?
A: Some kinds of cells reminiscent of hepatocytes carries enriched Albumin, and these liver derived exosomes will be launched into the circulation system. So, if the exosomes from these sorts of samples are remoted, it might accommodates Albumin.

8, Can I take advantage of your PureExo equipment (Cat. #P100) on our D.discoideum amoeba?
A: PureExo exosome isolation equipment captures launched exosomes or microvesicles from cells. For amoeba exosome isolation, we advise to gather amoeba tradition medium as the identical technique to gather cell tradition medium.

9, What’s the composition of the buffer that the ultimate exosomes prep can be in? Will it affect my downstream assay?
A: The ultimate remoted exosomes are aggregated nano-particles combined with a really small quantity of authentic tradition medium or serum. PureExo Exosome Isolation equipment offers intact exosomes which permits for a types of downstream purposes together with exosome floor labeling10， How have you learnt that an exosome prep is pure, freed from different membrane-derived microparticles
(shredding vesicles)?
A: The remoted microvesicle from cell tradition medium or serum utilizing our isolation kits confirmed sphere membrane encapsulated particles with the diameters various between 20-200nm underneath EM scanning. These characters assist to find out that the harvested microvesicle are exosomes. Shedding particles are reported to be irregular form with diameter as much as 1000nm. As well as, the scale distribution sample is confirmed by dynamic mild scattering indicating the remoted microvesicles are of diameter between 100 and 250nm.

11， Are exosome functionally lively?
A: Our collaborators used our kits to detect particular mirRNAs carried by exosomes. Luciferase exercise assay
confirmed lively perform of exosomal microRNA after the exosomes have been utilized to cells transfected with focusing on mRNA 3’UTR vector.

12， Is it crucial to make use of glass tubes?
A: Utilizing glass tubes as a substitute of plastic tubes is required in step Three and step 4.
Please discover glass tubes from FisherScientific. http://www.fishersci.com (Seek for Fisherbrand* Disposable
Borosilicate Glass Tubes Cat. NO: 14-961-26).

13， Why can’t I see clear fluff center layer?
A: The next dealing with errors might result in the failure:
1). Answer A/B/C didn’t combine utterly earlier than including the cell tradition medium or serum.
2). Didn’t carry out a low velocity centrifugation to take away cells and cell particles.
3). The ratio is off. For instance: 0.125 mL A + 0.125 mL B + 0.5mL C to 2 mL medium. Overproportion of serum/medium quantity to Answer combination (A/B/C) will result in an indistinctive interphase.
4). The utmost beginning quantity is Four ml medium, and it must be from half million cultured cells or much less. If cell quantity or medium quantity exceed this quantity, the pattern must be splits into a number of samples to proceed.